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Inhibition of SNARE-mediated membrane traffic impairs cell migration
- Source :
- Experimental Cell Research. 305:63-73
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- Cell migration occurs as a highly-regulated cycle of cell polarization, membrane extension at the leading edge, adhesion, contraction of the cell body, and release from the extracellular matrix at the trailing edge. In this study, we investigated the involvement of SNARE-mediated membrane trafficking in cell migration. Using a dominant-negative form of the enzyme N-ethylmaleimide-sensitive factor as a general inhibitor of SNARE-mediated membrane traffic and tetanus toxin as a specific inhibitor of VAMP3/cellubrevin, we conducted transwell migration assays and determined that serum-induced migration of CHO-K1 cells is dependant upon SNARE function. Both VAMP3-mediated and VAMP3-independent traffic were involved in regulating this cell migration. Inhibition of SNARE-mediated membrane traffic led to a decrease in the protrusion of lamellipodia at the leading edge of migrating cells. Additionally, the reduction in cell migration resulting from the inhibition of SNARE function was accompanied by perturbation of a Rab11-containing alpha(5)beta(1) integrin compartment and a decrease in cell surface alpha(5)beta(1) without alteration to total cellular integrin levels. Together, these observations suggest that inhibition of SNARE-mediated traffic interferes with the intracellular distribution of integrins and with the membrane remodeling that contributes to lamellipodial extension during cell migration.
- Subjects :
- Integrins
Integrin
Cell
Vesicular Transport Proteins
CHO Cells
Transfection
Extracellular matrix
Cell Movement
Cricetinae
Cell polarity
Cell Adhesion
medicine
Animals
N-Ethylmaleimide-Sensitive Proteins
VAMP3
biology
Cell Membrane
Cell Polarity
Cell migration
Cell Biology
Recombinant Proteins
Fibronectins
Cell biology
medicine.anatomical_structure
Amino Acid Substitution
Mutagenesis, Site-Directed
biology.protein
Lamellipodium
SNARE Proteins
Intracellular
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 305
- Database :
- OpenAIRE
- Journal :
- Experimental Cell Research
- Accession number :
- edsair.doi.dedup.....076e5d6f9bf5ab8b4eb3286e1a7ef1fb