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In vitro Entamoeba histolytica adhesion to human endothelium: a comparison using two strains of different virulence
- Source :
- Parasitology Research. 83:397-400
- Publication Year :
- 1997
- Publisher :
- Springer Science and Business Media LLC, 1997.
-
Abstract
- Extraintestinal dissemination of Entamoeba histolytica is frequently manifested by the life-threatening amebic liver abscess (ALA). The hepatic establishment of amebas implies invasion of blood vessels and contact with the endothelium. By means of a fluorescence-based quantitative adhesion assay, we assessed the binding to human endothelial cells of two E. histolytica strains of different virulence. The highly virulent strain (L-A) adhered substantially more strongly to unstimulated endothelium than the non-virulent one (BG3). Attachment of L-A was increased by treatment of endothelial cells with interleukin-1 beta (IL1 beta). Other proinflammatory cytokines such as interferon-gamma (IFN gamma) and tumor necrosis factor-alpha (TNF alpha) did not modify the spontaneous adhesion capacity of amebas. For purposes of comparison we also performed adhesion of the parasites to skin fibroblasts. Adhesion to this cell type was quite low (< 10%). Parasite virulence, differential adhesive capacity to endothelial cells, and modulation of the latter phenomenon by proinflammatory factors (IL1 beta) may influence the evolution and outcome of extraintestinal amebiasis, especially hepatic abscesses.
- Subjects :
- Endothelium
Virulence
Biology
Microbiology
Proinflammatory cytokine
Entamoeba histolytica
parasitic diseases
Cell Adhesion
medicine
Animals
Humans
Cell adhesion
Skin
General Veterinary
Genetic Variation
General Medicine
Adhesion
Fibroblasts
biology.organism_classification
In vitro
Infectious Diseases
medicine.anatomical_structure
Insect Science
Cytokines
Parasitology
Tumor necrosis factor alpha
Endothelium, Vascular
Subjects
Details
- ISSN :
- 14321955 and 09320113
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Parasitology Research
- Accession number :
- edsair.doi.dedup.....0777513c596cb3dcbcd77bd741b4e80c