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Whole Genome Sequencing and Comparative Genomic Analyses of Lysinibacillus pakistanensis LZH-9, a Halotolerant Strain with Excellent COD Removal Capability
- Source :
- Microorganisms, Microorganisms, Vol 8, Iss 716, p 716 (2020), Volume 8, Issue 5
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- Halotolerant microorganisms are promising in bio-treatment of hypersaline industrial wastewater. Four halotolerant bacteria strains were isolated from wastewater treatment plant, of which a strain LZH-9 could grow in the presence of up to 14% (w/v) NaCl, and it removed 81.9% chemical oxygen demand (COD) at 96 h after optimization. Whole genome sequencing of Lysinibacillus pakistanensis LZH-9 and comparative genomic analysis revealed metabolic versatility of different species of Lysinibacillus, and abundant genes involved in xenobiotics biodegradation, resistance to toxic compound, and salinity were found in all tested species of Lysinibacillus, in which Horizontal Gene Transfer (HGT) contributed to the acquisition of many important properties of Lysinibacillus spp. such as toxic compound resistance and osmotic stress resistance as revealed by phylogenetic analyses. Besides, genome wide positive selection analyses revealed seven genes that contained adaptive mutations in Lysinibacillus spp., most of which were multifunctional. Further expression assessment with Codon Adaption Index (CAI) also reflected the high metabolic rate of L. pakistanensis to digest potential carbon or nitrogen sources in organic contaminants, which was closely linked with efficient COD removal ability of strain LZH-9. The high COD removal efficiency and halotolerance as well as genomic evidences suggested that L. pakistanensis LZH-9 was promising in treating hypersaline industrial wastewater.
- Subjects :
- Microbiology (medical)
Microorganism
halotolerance
Lysinibacillus spp
comparative genomics
Biology
Microbiology
Genome
Article
03 medical and health sciences
Virology
Food science
lcsh:QH301-705.5
030304 developmental biology
Comparative genomics
Whole genome sequencing
0303 health sciences
Lysinibacillus
COD removal capability
030306 microbiology
Chemical oxygen demand
lcsh:Biology (General)
Horizontal gene transfer
Halotolerance
horizontal gene transfer
Subjects
Details
- Language :
- English
- ISSN :
- 20762607
- Volume :
- 8
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....079b96e456d1f912fec56ef8f6ca244a