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MircoRNA-145 promotes activation of hepatic stellate cells via targeting krüppel-like factor 4
- Source :
- Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- Krüppel-like Factor 4 (KLF4), a target gene of miR-145, can negatively regulate lung fibrosis. However, the potential role of KLF4 and miR-145 in hepatic stellate cells (HSCs) activation or in hepatic fibrosis keeps unclear. This study aims to characterize miR-145 and KLF4 in activated HSCs and liver cirrhotic, and the underlying molecular basis. miR-145 was significantly up-regulated, while KLF4 was dramatically down-regulated during the activation of rat primary HSCs and TGF-βtreated HSCs. Furthermore, miR-145 mimics induced and inhibition of miR-145 reduced α-SMA and COL-I expression in primary HSCs. Additionally, the mRNA and protein levels of KLF4 in the liver of cirrhotic patients and rats were significantly down-regulated. α-SMA and COL-I were increased after inhibition of KLF4 by specific shRNA in primary HSCs. Forced KLF4 expression led to a reduction of α-SMA and COL-I expression in HSCs. miR-145 promotes HSC activation and liver fibrosis by targeting KLF4.
- Subjects :
- Liver Cirrhosis
Male
0301 basic medicine
Kruppel-Like Transcription Factors
Down-Regulation
Article
Rats, Sprague-Dawley
Small hairpin RNA
Kruppel-Like Factor 4
Mice
03 medical and health sciences
stomatognathic system
Downregulation and upregulation
Transforming Growth Factor beta
microRNA
Hepatic Stellate Cells
Animals
Humans
Messenger RNA
Multidisciplinary
biology
Chemistry
fungi
Transforming growth factor beta
Up-Regulation
Cell biology
MicroRNAs
030104 developmental biology
KLF4
Gene Knockdown Techniques
embryonic structures
Hepatic stellate cell
biology.protein
sense organs
biological phenomena, cell phenomena, and immunity
Hepatic fibrosis
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....07a2e8e3026c92d6357a07a83f68485f
- Full Text :
- https://doi.org/10.1038/srep40468