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Regulation of reactive oxygen species and stress fiber formation by calpeptin in Swiss 3T3 fibroblasts
- Source :
- Cellular signalling. 14(3)
- Publication Year :
- 2002
-
Abstract
- We have investigated a novel function of calpeptin, a commonly used inhibitor of calpain, in the production of intracellular reactive oxygen species (ROS) in Swiss 3T3 fibroblasts. Calpeptin induced a rapid increase of intracellular ROS by a dose-dependent manner, with a maximal increase at 10 min, which was inhibited by ROS scavengers, catalase and 2-MPG. However, other calpain inhibitors, E64d and N-acetyl-Leu-Leu-Nle-CHO (ALLN), had no effect on the level of intracellular ROS, indicating that calpain was not involved in the ROS production by calpeptin. The role of Rho in the ROS production by calpain was studied by scrape-loading of C3 transferase. C3 transferase, which inhibited stress fiber formation by calpeptin, had no effect on the ROS production in response to calpeptin, suggesting that Rho was not involved in the ROS production by calpeptin. But the elevation of intracellular ROS was inhibited by mepacrine, a phospholipase A2 inhibitor. In addition, scavenging intracellular ROS by the incubation with catalase and 2-MPG had no effect on the stress fiber formation by calpeptin. These results suggested that calpeptin stimulated the production of intracellular ROS and stress fiber formation by independent mechanisms.
- Subjects :
- chemistry.chemical_classification
Intracellular Fluid
rho GTP-Binding Proteins
Reactive oxygen species
Stress fiber
Calpain
Ros scavenger
Cell Biology
3T3 Cells
Dipeptides
Biology
Cysteine Proteinase Inhibitors
Phospholipases A
Cell biology
C3 transferase
Mice
Phospholipases A2
chemistry
Catalase
biology.protein
Animals
Reactive Oxygen Species
Incubation
Intracellular
Subjects
Details
- ISSN :
- 08986568
- Volume :
- 14
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Cellular signalling
- Accession number :
- edsair.doi.dedup.....07d375a00edba62389bd67b157fd1da0