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Colorimetric detection of Staphylococcus aureus using gold nanorods labeled with yolk immunoglobulin and urease, magnetic beads, and a phenolphthalein impregnated test paper
- Source :
- Mikrochimica acta. 186(9)
- Publication Year :
- 2019
-
Abstract
- A colorimetric test is described for the rapid detection of Staphylococcus aureus (SA). Gold nanorods (AuNRs) are first labeled with urease and yolk immunoglobulin (IgY). This probe can specifically bind SA. In the next step, nonspecific magnetic beads and sample are added. This leads to the formation of the AuNR-IgY-SA-nMB immunocomplex which is then magnetically separated. Finally, a solution of urea is added to the supernatant. Ureases catalyzes the decomposition of urea which results in an increase in the pH value. The increase in the pH value is detected by using a phenolphthalein test paper which undergoes a color change from white to pink. The analytical process can be completed within 20 min. The method is highly specific and can detect as little as 476 cfu·mL−1. It was verified by analyzing contaminated Chinese cabbage and beef samples, and 1000 cfu·mL−1 of SA were accurately detected.
- Subjects :
- Paper
Staphylococcus aureus
Urease
Magnetic separation
Nanoparticle
Immunoglobulins
02 engineering and technology
Biosensing Techniques
Phenolphthalein
medicine.disease_cause
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
medicine
Chromatography
Nanotubes
medicine.diagnostic_test
biology
010401 analytical chemistry
021001 nanoscience & nanotechnology
Egg Yolk
Microspheres
0104 chemical sciences
chemistry
Immunoassay
Urea
biology.protein
Magnets
Nanorod
Colorimetry
Gold
0210 nano-technology
Subjects
Details
- ISSN :
- 14365073
- Volume :
- 186
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Mikrochimica acta
- Accession number :
- edsair.doi.dedup.....07fab9223de4c5338449f4f82ec69b72