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A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination
- Source :
- Vaccines, Vol 9, Iss 418, p 418 (2021), IRTA Pubpro. Open Digital Archive, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Vaccines, Volume 9, Issue 5
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- The efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced in plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A Nicotiana benthamiana tissue culture system was used to obtain a stable production of the E2- glycoprotein fused to the porcine Fc region of IgG. Ten pregnant sows were divided into three groups: Groups 1 and 2 (four sows each) were vaccinated with either 100 µg/dose or 300 µg/dose of the subunit vaccine at 64 days of pregnancy. Group 3 (two sows) was injected with PBS. Groups 1 and 2 were boosted with the same vaccine dose. At 10 days post second vaccination, the sows in Groups 2 and 3 were challenged with a highly virulent CSFV strain. The vaccinated sows remained clinically healthy and seroconverted rapidly, showing efficient neutralizing antibodies. The fetuses from vaccinated sows did not show gross lesions, and all analyzed tissue samples tested negative for CSFV replication. However, fetuses of non-vaccinated sows had high CSFV replication in tested tissue samples. The results suggested that in vaccinated sows, the plant produced E2 marker vaccine induced the protective immunogenicity at challenge, leading to protection from vertical transmission to fetuses. info:eu-repo/semantics/publishedVersion
- Subjects :
- 0301 basic medicine
040301 veterinary sciences
animal diseases
Immunology
Nicotiana benthamiana
Virulence
plant
Marker vaccine
Biology
classical swine fever virus
Article
Virus
0403 veterinary science
03 medical and health sciences
Drug Discovery
Pharmacology (medical)
Pharmacology
marker vaccine
Immunogenicity
food and beverages
04 agricultural and veterinary sciences
biology.organism_classification
protection
Virology
Vaccination
030104 developmental biology
Infectious Diseases
E2 glycoprotein
Classical swine fever
biology.protein
Medicine
vertical transmission
Antibody
Subjects
Details
- Language :
- English
- Volume :
- 9
- Issue :
- 418
- Database :
- OpenAIRE
- Journal :
- Vaccines
- Accession number :
- edsair.doi.dedup.....07ff2bb4dc6567d3238a40a919548f13