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Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression-A Mouse Study
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences; Volume 18; Issue 11; Pages: 2467, International Journal of Molecular Sciences, Vol 18, Iss 11, p 2467 (2017)
- Publication Year :
- 2017
-
Abstract
- chlorpyrifos (CPF) is an organophosphate insecticide used to control pests on a variety of food and feed crops. In mammals, maternal exposure to CPF has been reported to induce cerebral cortex thinning, alteration of long-term brain cognitive function, and Parkinson-like symptoms, but the mechanisms of these processes are not fully understood. In this study, we aimed to gain a deeper understanding of the alterations induced in the brains of mice chronically exposed to CPF by dietary intake. For our purpose, we analysed F1 offspring (sacrificed at 3 and 8 months) of Mus musculus, treated in utero and postnatally with 3 different doses of CPF (0.1-1-10 mg/kg/day). Using RT² Profiler PCR Arrays, we evaluated the alterations in the expression of 84 genes associated with neurodegenerative diseases. In the brains of exposed mice, we evidenced a clear dose-response relationship for AChE inhibition and alterations of gene expression. Some of the genes that were steadily down-regulated, such as Pink1, Park 2, Sv2b, Gabbr2, Sept5 and Atxn2, were directly related to Parkinson's onset. Our experimental results shed light on the possibility that long-term CPF exposure may exert membrane signalling alterations which make brain cells more susceptible to develop neurodegenerative diseases.
- Subjects :
- 0301 basic medicine
Insecticides
Parkinson's disease
chlorpyrifos
organophosphate insecticides
Mus musculus
developmental neurotoxicity
Parkinson’s disease
neurodegenerative diseases
PCR-array
Mus musculu
lcsh:Chemistry
Dietary Exposure
chemistry.chemical_compound
Mice
0302 clinical medicine
neurodegenerative disease
Gene expression
lcsh:QH301-705.5
Spectroscopy
Organophosphate
chlorpyrifo
Brain
General Medicine
Computer Science Applications
medicine.anatomical_structure
In utero
Cerebral cortex
Maternal Exposure
language
Female
medicine.medical_specialty
Aché
Offspring
PINK1
Biology
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Internal medicine
medicine
Animals
Humans
Physical and Theoretical Chemistry
Parkinson Disease, Secondary
Molecular Biology
business.industry
Organic Chemistry
medicine.disease
language.human_language
organophosphate insecticide
Biotechnology
030104 developmental biology
Endocrinology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
Protein Biosynthesis
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 18
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.doi.dedup.....08053393c9fb6dceb0a686e5afd4dc5a