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Serotype IX, a Proposed New Streptococcus agalactiae Serotype
- Source :
- Journal of Clinical Microbiology. 45:2929-2936
- Publication Year :
- 2007
- Publisher :
- American Society for Microbiology, 2007.
-
Abstract
- We identified three isolates of Streptococcus agalactiae (group B streptococcus [GBS]), of human origin, which failed to react with antisera against any of the nine known GBS serotypes. Polyclonal rabbit antisera raised against these isolates and standard GBS typing sera were used in capillary precipitation and Ouchterlony tests to compare the strains with known GBS serotype reference strains. All three previously nontypeable isolates reacted with all three new antisera, producing lines of identity in the Ouchterlony test. Weak cross-reactions with antisera against several GBS serotypes were observed but were removed by absorption with corresponding antigens. The new antisera were used to test 227 GBS isolates that had been nontypeable or difficult to type using standard antisera. Of these, five reacted with the new antisera. These results suggested that all eight isolates belong to the previously unrecognized GBS serotype. They were tested by Western blotting for the Cα and Cβ proteins and by PCR to identify molecular serotypes and surface protein antigen genes. Two segments of the cps gene cluster (3′ end of cpsE - cpsF and 5′ end of cpsG , approximately 700 bp; 3′ end of cpsH and 5′ end of cpsM , approximately 560 bp) were sequenced. All eight isolates expressed Cα, and seven expressing the Cβ protein and the corresponding genes, bca and bac , respectively, were identified. They all share the same, unique partial cps sequence. These results indicate that these eight isolates represent a new S. agalactiae serotype, which we propose should be designated serotype IX.
- Subjects :
- Adult
DNA, Bacterial
Male
Microbiology (medical)
Serotype
Sequence analysis
Blotting, Western
Molecular Sequence Data
Cross Reactions
medicine.disease_cause
Polymerase Chain Reaction
Streptococcus agalactiae
Microbiology
Bacterial Proteins
Pregnancy
Streptococcal Infections
medicine
Humans
Immunoprecipitation
Amino Acid Sequence
Typing
Serotyping
Antiserum
Base Sequence
biology
Streptococcus
Infant, Newborn
Membrane Proteins
Bacteriology
Sequence Analysis, DNA
Middle Aged
Ouchterlony double immunodiffusion
Virology
Polyclonal antibodies
biology.protein
Female
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....082098e003763f88d042223929edd220