Supplementary Figure 6 from Combined EGFR/MEK Inhibition Prevents the Emergence of Resistance in EGFR-Mutant Lung Cancer

Supplementary Figure S6. Resistance to W+T combination treatment. (A) Individual tumor volume beyond 20 weeks of WZ4002 + trametinib co-treatment. (B) Representative MRI image of tumor rebound. (C) Immunohistochemical analysis of WZ4002 + trametinib combination resistant tumors demonstrating positive staining for phospho-EGFR, ERK1/2, S6 and 4EBP1 at 50 nM (left panel) and 100 nM (right panel). (D) Immunoblot analysis of EGFR pathway reactivation in WZ4002 + trametinib resistant LT GEMMs. Phospho-EGFR, AKT, ERK1/2, S6 and 4EBP1 were assessed. Lysates from LT GEMMs were treated with vehicle or with WZ4002 for 24 hours were included as positive and negative controls for EGFR pathway activation.