Back to Search
Start Over
The Retinal Ganglion Cell Response to Blast-Mediated Traumatic Brain Injury Is Genetic Background Dependent
- Source :
- Investigative Ophthalmology & Visual Science
- Publication Year :
- 2021
-
Abstract
- Purpose The purpose of this study was to examine the influence of genetic background on the retinal ganglion cell (RGC) response to blast-mediated traumatic brain injury (TBI) in Jackson Diversity Outbred (J:DO), C57BL/6J and BALB/cByJ mice. Methods Mice were subject to one blast injury of 137 kPa. RGC structure was analyzed by optical coherence tomography (OCT), function by the pattern electroretinogram (PERG), and histologically using BRN3A antibody staining. Results Comparison of the change in each group from baseline for OCT and PERG was performed. There was a significant difference in the J:DOΔOCT compared to C57BL/6J mice (P = 0.004), but not compared to BALB/cByJ (P = 0.21). There was a significant difference in the variance of the ΔOCT in J:DO compared to both C57BL/6J and BALB/cByJ mice. The baseline PERG amplitude was 20.33 ± 9.32 µV, which decreased an average of -4.14 ± 12.46 µV following TBI. Baseline RGC complex + RNFL thickness was 70.92 ± 4.52 µm, which decreased an average of -1.43 ± 2.88 µm following blast exposure. There was not a significant difference in the ΔPERG between J:DO and C57BL/6J (P = 0.13), although the variances of the groups were significantly different. Blast exposure in J:DO mice results in a density change of 558.6 ± 440.5 BRN3A-positive RGCs/mm2 (mean ± SD). Conclusions The changes in retinal outcomes had greater variance in outbred mice than what has been reported, and largely replicated herein, for inbred mice. These results demonstrate that the RGC response to blast injury is highly dependent upon genetic background.
- Subjects :
- 0301 basic medicine
Retinal Ganglion Cells
medicine.medical_specialty
vision
genetic structures
neurotrauma
Traumatic brain injury
Blast exposure
blast
pattern electroretinogram
Blast injury
Retina
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Blast Injuries
Stress, Physiological
Ophthalmology
Brain Injuries, Traumatic
medicine
Electroretinography
Animals
Mice, Inbred BALB C
Transcription Factor Brn-3A
business.industry
traumatic brain injury
Significant difference
Genetic Variation
Retinal
General Medicine
medicine.disease
Density change
Immunohistochemistry
eye diseases
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Retinal ganglion cell
chemistry
sense organs
Antibody staining
business
030217 neurology & neurosurgery
Tomography, Optical Coherence
Subjects
Details
- ISSN :
- 15525783
- Volume :
- 62
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Investigative ophthalmologyvisual science
- Accession number :
- edsair.doi.dedup.....0839545830575de86587ca9b5c8e709e