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Comparisons of Expression Levels of Heat Shock Proteins (hsp70 and hsp90) From Anaphothrips obscurus (Thysanoptera: Thripidae) in Polymorphic Adults Exposed to Different Heat Shock Treatments
- Source :
- Journal of Insect Science
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Heat shock proteins (Hsps) are prominent proteins that greatly contribute to insect survival under stress conditions. In this study, we cloned two Hsp transcripts (Aohsp70 and Aohsp90) from the grass thrip, Anaphothrips obscurus (Müller) (Thysanoptera: Thripidae), which is a polymorphic winged pest of corn and wheat. The cDNA sequences of Aohsp70 and Aohsp90 are 2382 and 2504 bp long, and encode proteins with calculated molecular weights of 70.02 kDa and 83.40 kDa, respectively. Aohsp90 was highly expressed in adults of both brachypters and macropters. Aohsp70 had different expression patterns in brachypters and macropters and was also highly expressed in the pupae of macropters. After adults were exposed to an ascending series of heat shocks, the expression of both Aohsp70 and Aohsp90 were up-regulated. In macropters and brachypters, the maximum induced levels of Aohsp70 (approximately 90-fold and 280-fold, respectively) were higher than Aohsp90 (approximately 2.4-fold and 1.8-fold, respectively). In addition, the up-regulation of Aohsp70 was significantly higher in brachypters than in macropters. Brachypters had a significantly higher Ltem50 (43.2°C) than macropters (42.5°C), which implied that brachypters are more tolerant to thermal stress than macropters. This study has shown that the expression patterns of Aohsp70 and Aohsp90 are variable among different life stages and thermal stress induced different levels of expressions in macropterous and brachypterous adults.
- Subjects :
- Nymph
0301 basic medicine
Hot Temperature
media_common.quotation_subject
heat shock protein
Zoology
Insect
03 medical and health sciences
Heat shock protein
Complementary DNA
Animals
HSP70 Heat-Shock Proteins
Amino Acid Sequence
HSP90 Heat-Shock Proteins
Phylogeny
media_common
Base Sequence
biology
Thysanoptera
Pupa
Gene Expression Regulation, Developmental
General Medicine
Thripidae
biology.organism_classification
Hsp90
thermal stress
Hsp70
030104 developmental biology
Insect Science
biology.protein
wing polymorphism
Insect Proteins
PEST analysis
thrip
Research Article
Subjects
Details
- ISSN :
- 15362442
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Insect Science
- Accession number :
- edsair.doi.dedup.....08453075acb8de6d87be4f9176668cff