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Small Red Blood Cell Fraction on the UF-1000i Urine Analyzer as a Screening Tool to Detect Dysmorphic Red Blood Cells for Diagnosing Glomerulonephritis
- Source :
- Annals of Laboratory Medicine
- Publication Year :
- 2018
-
Abstract
- Background Dysmorphic red blood cells (dRBCs) are first-line biomarkers for detecting glomerulonephritis (GN) in patients with hematuria. The UF-1000i system (Sysmex, Kobe, Japan), based on flow cytometry, provides small red blood cell (RBC) values (UF-1000i [UF]-%sRBCs). We evaluated the clinical application of UF-%sRBCs for detecting %dRBCs and GN. Methods Urine samples of 103 patients (47 with GN; 56 without GN [NGN]) were analyzed using UF-1000i urinalysis, phase-contrast microscopy (PCM), and urine chemistry. Serum creatinine (mg/dL), serum albumin (g/dL), serum protein (mg/dL), urine protein (mg/dL), and urea nitrogen (mg/dL) levels were measured using an automated chemical analyzer. To determine the cut-off level of predicting GN, ROC curve was analyzed. Results UF-%sRBCs, %dRBCs, urine protein, serum creatinine, and estimated-glomerular filtration rate differed between the GN and NGN groups, with the greatest differences detected for UF-%sRBCs and %dRBCs (P40.5% and >6.7%, respectively. %dRBCs (P=0.0001) and UF-%sRBCs (P=0.0006) differed between the GN and NGN groups in patients with isolated hematuria but without proteinuria. Conclusions UF-%sRBCs had similar diagnostic power to %dRBCs determined by PCM for identifying patients with GN. UF-%sRBCs may be more useful for diagnosing GN in patients with isolated hematuria. Predicting %dRBCs using UF-1000i will provide information on possible GN in patients presenting with asymptomatic hematuria.
- Subjects :
- Male
Erythrocytes
Clinical Biochemistry
030232 urology & nephrology
Urine
UF-1000i
Gastroenterology
chemistry.chemical_compound
0302 clinical medicine
Glomerulonephritis
Medicine
Small red blood cell
Clinical Chemistry
Proteinuria
biology
medicine.diagnostic_test
General Medicine
Middle Aged
Flow Cytometry
medicine.anatomical_structure
Area Under Curve
Creatinine
Screening
030211 gastroenterology & hepatology
Female
Original Article
medicine.symptom
Glomerular Filtration Rate
Adult
medicine.medical_specialty
Urinalysis
Serum albumin
Renal function
Sensitivity and Specificity
03 medical and health sciences
Dysmorphic red blood cell
Internal medicine
Humans
Aged
Hematuria
business.industry
Biochemistry (medical)
medicine.disease
Red blood cell
chemistry
ROC Curve
Case-Control Studies
biology.protein
business
Subjects
Details
- ISSN :
- 22343814
- Volume :
- 39
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Annals of laboratory medicine
- Accession number :
- edsair.doi.dedup.....0871b060ea4011d499e232b540a210dd