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Breast Cancer Cell Line Phenotype Affects Sonoporation Efficiency Under Optimal Ultrasound Microbubble Conditions
- Source :
- Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
- Publication Year :
- 2018
- Publisher :
- International Scientific Literature, Inc., 2018.
-
Abstract
- BACKGROUND Ultrasound/microbubble (USMB)-mediated sonoporation is a new strategy with minimal procedural invasiveness for targeted and site-specific drug delivery to tumors. The purpose of this study was to explore the effect of different breast cancer cell lines on sonoporation efficiency, and then to identify an optimal combination of USMB parameters to maximize the sonoporation efficiency for each tumor cell line. MATERIAL AND METHODS Three drug-sensitive breast cell lines - MCF-7, MDA-MB-231, and MDA-MB-468 - and 1 multidrug resistance (MDR) cell line - MCF-7/ADR - were chosen. An orthogonal array experimental design approach based on 3 levels of 3 parameters (A: microbubble concentration, 10%, 20%, and 30%, B: sound intensity, 0.5, 1.0, and 1.5 W/cm², C: irradiation time, 30, 60, and 90 s) was employed to optimize the sonoporation efficiency. RESULTS The optimal USMB parameter combinations for different cell lines were diverse. Under optimal parameter combinations, the maximum sonoporation efficiency differences between different breast tumor cell lines were statistically significant (MDA-MB-231: 46.70±5.79%, MDA-MB-468: 53.44±5.69%, MCF-7: 59.88±5.53%, MCF-7/ADR: 65.39±4.01%, P
- Subjects :
- Ultrasonic Therapy
Triple Negative Breast Neoplasms
Breast Neoplasms
02 engineering and technology
Irradiation time
03 medical and health sciences
0302 clinical medicine
Drug Delivery Systems
Breast cancer cell line
Lab/In Vitro Research
Cell Line, Tumor
medicine
Neoplasm
Humans
skin and connective tissue diseases
Ultrasonography
Microbubbles
business.industry
Ultrasound
General Medicine
021001 nanoscience & nanotechnology
medicine.disease
Phenotype
Ultrasonic Waves
Cell culture
Drug Resistance, Neoplasm
Research Design
030220 oncology & carcinogenesis
Drug delivery
Cancer research
MCF-7 Cells
Female
Genes, MDR
0210 nano-technology
business
Sonoporation
Subjects
Details
- Language :
- English
- ISSN :
- 16433750 and 12341010
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
- Accession number :
- edsair.doi.dedup.....08958426beca9285d927288dc35a5a72