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Selective regulation of lymphopoiesis and leukemogenesis by individual zinc fingers of Ikaros
- Source :
- Nature Immunology. 14:1073-1083
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- C2H2 zinc fingers are found in several key transcriptional regulators in the immune system. However, these proteins usually contain more fingers than are needed for sequence-specific DNA binding, which suggests that different fingers regulate different genes and functions. Here we found that mice lacking finger 1 or finger 4 of Ikaros exhibited distinct subsets of the hematological defects of Ikaros-null mice. Most notably, the two fingers controlled different stages of lymphopoiesis, and finger 4 was selectively required for tumor suppression. The distinct defects support the hypothesis that only a small number of genes that are targets of Ikaros are critical for each of its biological functions. The subcategorization of functions and target genes by mutagenesis of individual zinc fingers will facilitate efforts to understand how zinc-finger transcription factors regulate development, immunity and disease.
- Subjects :
- Chromatin Immunoprecipitation
Lymphoma
Molecular Sequence Data
Immunology
Fusion Proteins, bcr-abl
Mutagenesis (molecular biology technique)
Biology
Immunophenotyping
Ikaros Transcription Factor
Mice
03 medical and health sciences
0302 clinical medicine
Animals
Cluster Analysis
Position-Specific Scoring Matrices
Immunology and Allergy
Lymphopoiesis
Nucleotide Motifs
Transcription factor
Germ-Line Mutation
030304 developmental biology
Mice, Knockout
Regulation of gene expression
Genetics
Zinc finger
B-Lymphocytes
0303 health sciences
Binding Sites
Leukemia
Thymocytes
Base Sequence
C2H2 Zinc Finger
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Cell Differentiation
3. Good health
body regions
Cell Transformation, Neoplastic
Phenotype
Gene Expression Regulation
030220 oncology & carcinogenesis
Chromatin immunoprecipitation
Protein Binding
Subjects
Details
- ISSN :
- 15292916 and 15292908
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Nature Immunology
- Accession number :
- edsair.doi.dedup.....08aed491927e168aaf74cfdc8a6d5136