Back to Search
Start Over
Comprehensive assessment of post-prandial protein handling by the application of intrinsically labelled protein in vivo in human subjects
- Source :
- Proceedings of the Nutrition Society, 80(2):0029665120008034, 221-229. Cambridge University Press
- Publication Year :
- 2021
-
Abstract
- All human tissues are in a constant state of remodelling, regulated by the balance between tissue protein synthesis and breakdown rates. It has been well-established that protein ingestion stimulates skeletal muscle and whole-body protein synthesis. Stable isotope-labelled amino acid methodologies are commonly applied to assess the various aspects of protein metabolism in vivo in human subjects. However, to achieve a more comprehensive assessment of post-prandial protein handling in vivo in human subjects, intravenous stable isotope-labelled amino acid infusions can be combined with the ingestion of intrinsically labelled protein and the collection of blood and muscle tissue samples. The combined application of ingesting intrinsically labelled protein with continuous intravenous stable isotope-labelled amino acid infusion allows the simultaneous assessment of protein digestion and amino acid absorption kinetics (e.g. release of dietary protein-derived amino acids into the circulation), whole-body protein metabolism (whole-body protein synthesis, breakdown and oxidation rates and net protein balance) and skeletal muscle metabolism (muscle protein fractional synthesis rates and dietary protein-derived amino acid incorporation into muscle protein). The purpose of this review is to provide an overview of the various aspects of post-prandial protein handling and metabolism with a focus on insights obtained from studies that have applied intrinsically labelled protein under a variety of conditions in different populations.
- Subjects :
- 0301 basic medicine
Muscle tissue
Protein digestion
POSTEXERCISE RECOVERY
Protein metabolism
Medicine (miscellaneous)
030209 endocrinology & metabolism
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Protein quality
SYNTHESIS RATES
medicine
Protein biosynthesis
AMINO-ACIDS
RESISTANCE EXERCISE
chemistry.chemical_classification
030109 nutrition & dietetics
Nutrition and Dietetics
Chemistry
Skeletal muscle
DIETARY-PROTEIN
Metabolism
Hypertrophy
MUSCLE
Amino acid
medicine.anatomical_structure
Biochemistry
Anabolism
Digestibility
YOUNG
SYNTHETIC RESPONSE
MILK PROTEIN
INGESTION
Subjects
Details
- Language :
- English
- ISSN :
- 00296651
- Volume :
- 80
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Proceedings of the Nutrition Society
- Accession number :
- edsair.doi.dedup.....08b3ab354120d207cb21575e722fec41
- Full Text :
- https://doi.org/10.1017/s0029665120008034