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Proteasomal activator 28 gamma stabilizes hepatitis B virus X protein by competitively inhibiting the Siah-1-mediated proteasomal degradation
- Source :
- Biochemical and Biophysical Research Communications. 578:97-103
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Proteasomal activator 28 gamma (PA28γ) upregulates the levels of HBx, a regulatory protein of hepatitis B virus (HBV) to stimulate HBV replication; however, the detailed mechanism remains unknown. Here, we found that PA28γ impaired the ability of seven in absentia homolog 1 (Siah-1) as an E3 ubiquitin ligase of HBx. PA28γ competitively inhibited the binding of Siah-1 to HBx in human hepatoma cells. Accordingly, PA28γ increased the stability of HBx and decreased HBx ubiquitination, abolishing the potential of Siah-1 to downregulate HBx levels. PA28γ also executed its role as an antagonist of Siah-1 during HBV replication, as demonstrated by an in vitro HBV replication system. The present study may provide insights into the mechanisms underlying the regulation of HBV replication.
- Subjects :
- Hepatitis B virus
Proteasome Endopeptidase Complex
Ubiquitin-Protein Ligases
viruses
Biophysics
medicine.disease_cause
Autoantigens
Biochemistry
Ubiquitin
Downregulation and upregulation
Cell Line, Tumor
medicine
Humans
Viral Regulatory and Accessory Proteins
Molecular Biology
Regulation of gene expression
biology
Chemistry
Activator (genetics)
Liver Neoplasms
Ubiquitination
Nuclear Proteins
Cell Biology
Hepatitis B
digestive system diseases
Ubiquitin ligase
Cell biology
HBx
Proteasome
Trans-Activators
biology.protein
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 578
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....08bb2a22b2f393b7f87673e2b74d2eb4