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Ethyl acetate extract of Peperomia tetraphylla induces cytotoxicity, cell cycle arrest, and apoptosis in lymphoma U937 cells
- Source :
- Biomedicine & Pharmacotherapy. 84:1802-1809
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The current study evaluated the cytotoxicity and the mechanism of apoptotic induction by Peperomia tetraphylla in U937 lymphoma cells. The results showed that P. tetraphylla ethyl acetate extract (EAEPT) inhibited the cell growth in U937 cells by MTT assay. After the U937 cells were treated with EAEPT, the cells exhibited marked morphological features of apoptosis (Hoechst 33342 staining) and the number of apoptotic cell (Annexin V-FITC/PI staining) increased. The treatment of EAEPT could induce loss of mitochondrial membrane potential (MMP) and increase the ROS level. Moreover, EAEPT treatment resulted in the accumulation of cells at S phase. We found that EAEPT could induce the cleavage of the caspase 3, caspase 8, caspase 9 and Bid. And the treatment of EAEPT could increase expression of Bax and down-regulate the expression of CCNB1, CCND1 and CDK1. The sub-fraction of EAEPT, namely EASub1 demonstrated the highest cytotoxicity activity on U937 cells. It was confirmed that EAEPT could inhibit the growth of U937 cells by blocking the cell cycle and prompted apoptosis via the ROS-medicated mitochondria pathway in vitro.
- Subjects :
- 0301 basic medicine
Cell Survival
Peperomia tetraphylla
Apoptosis
Cell Cycle Proteins
Caspase 3
Acetates
Caspase 8
Peperomia
03 medical and health sciences
0302 clinical medicine
Humans
MTT assay
Cytotoxicity
Cell Proliferation
Membrane Potential, Mitochondrial
Pharmacology
Plants, Medicinal
Dose-Response Relationship, Drug
U937 cell
biology
Plant Extracts
Cell growth
Cell Cycle Checkpoints
U937 Cells
General Medicine
biology.organism_classification
Antineoplastic Agents, Phytogenic
Molecular biology
Cell biology
Oxidative Stress
030104 developmental biology
030220 oncology & carcinogenesis
Solvents
Lymphoma, Large B-Cell, Diffuse
Apoptosis Regulatory Proteins
Reactive Oxygen Species
Phytotherapy
Subjects
Details
- ISSN :
- 07533322
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Biomedicine & Pharmacotherapy
- Accession number :
- edsair.doi.dedup.....08c181c97144d54be9b52b049dd3852b