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Genetic Code Supports Targeted Insertion of Two Amino Acids by One Codon
- Source :
- Science. 323:259-261
- Publication Year :
- 2009
- Publisher :
- American Association for the Advancement of Science (AAAS), 2009.
-
Abstract
- Strict one-to-one correspondence between codons and amino acids is thought to be an essential feature of the genetic code. However, we report that one codon can code for two different amino acids with the choice of the inserted amino acid determined by a specific 3′ untranslated region structure and location of the dual-function codon within the messenger RNA (mRNA). We found that the codon UGA specifies insertion of selenocysteine and cysteine in the ciliate Euplotes crassus , that the dual use of this codon can occur even within the same gene, and that the structural arrangements of Euplotes mRNA preserve location-dependent dual function of UGA when expressed in mammalian cells. Thus, the genetic code supports the use of one codon to code for multiple amino acids.
- Subjects :
- DNA codon table
Recombinant Fusion Proteins
Molecular Sequence Data
Protozoan Proteins
Euplotes
Biology
Article
Cell Line
Start codon
Animals
Humans
Amino Acid Sequence
Cysteine
Codon degeneracy
Codon
Selenoproteins
3' Untranslated Regions
Expanded genetic code
Genetics
RNA, Transfer, Cys
Multidisciplinary
Base Sequence
RNA, Transfer, Amino Acid-Specific
Genetic code
Stop codon
Selenocysteine
Genetic Code
Codon usage bias
Mutation
Codon, Terminator
Synonymous substitution
RNA, Protozoan
Subjects
Details
- ISSN :
- 10959203 and 00368075
- Volume :
- 323
- Database :
- OpenAIRE
- Journal :
- Science
- Accession number :
- edsair.doi.dedup.....08e212d7030a5741f2ab65b668c49069
- Full Text :
- https://doi.org/10.1126/science.1164748