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Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level
- Source :
- Okholm, A H, Aslan, H, Besenbacher, F, Dong, MD & Kjems, J 2015, ' Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level ', Nanoscale, vol. 7, no. 25, pp. 10970-10973 . https://doi.org/10.1039/c5nr01945a
- Publication Year :
- 2015
-
Abstract
- DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.
- Subjects :
- chemistry.chemical_classification
Materials science
Atomic force microscopy
Surface Properties
Biomolecule
technology, industry, and agriculture
Nanotechnology
DNA
Microscopy, Atomic Force
Nanostructures
Polymerization
chemistry.chemical_compound
Enzyme
Terminal deoxynucleotidyl transferase
chemistry
DNA Nucleotidylexotransferase
Albumins
Molecule
DNA origami
General Materials Science
Biotechnology
Subjects
Details
- ISSN :
- 20403372
- Volume :
- 7
- Issue :
- 25
- Database :
- OpenAIRE
- Journal :
- Nanoscale
- Accession number :
- edsair.doi.dedup.....08ea53a034e5ee598c83f1f78729591d
- Full Text :
- https://doi.org/10.1039/c5nr01945a