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Genome-wide association study of periodontal pathogen colonization
Genome-wide association study of periodontal pathogen colonization
- Source :
- Journal of dental research. 91
- Publication Year :
- 2012
-
Abstract
- Pathological shifts of the human microbiome are characteristic of many diseases, including chronic periodontitis. To date, there is limited evidence on host genetic risk loci associated with periodontal pathogen colonization. We conducted a genome-wide association (GWA) study among 1,020 white participants of the Atherosclerosis Risk in Communities Study, whose periodontal diagnosis ranged from healthy to severe chronic periodontitis, and for whom “checkerboard” DNA-DNA hybridization quantification of 8 periodontal pathogens was performed. We examined 3 traits: “high red” and “high orange” bacterial complexes, and “high” Aggregatibacter actinomycetemcomitans (Aa) colonization. Genotyping was performed on the Affymetrix 6.0 platform. Imputation to 2.5 million markers was based on HapMap II-CEU, and a multiple-test correction was applied (genome-wide threshold of p < 5 × 10−8). We detected no genome-wide significant signals. However, 13 loci, including KCNK1, FBXO38, UHRF2, IL33, RUNX2, TRPS1, CAMTA1, and VAMP3, provided suggestive evidence (p < 5 × 10−6) of association. All associations reported for “red” and “orange” complex microbiota, but not for Aa, had the same effect direction in a second sample of 123 African-American participants. None of these polymorphisms was associated with periodontitis diagnosis. Investigations replicating these findings may lead to an improved understanding of the complex nature of host-microbiome interactions that characterizes states of health and disease.
- Subjects :
- DNA, Bacterial
Male
Periodontium
Vesicle-Associated Membrane Protein 3
Ubiquitin-Protein Ligases
Genome-wide association study
Core Binding Factor Alpha 1 Subunit
Biology
Aggregatibacter actinomycetemcomitans
Prevotella intermedia
Periodontal pathogen
Potassium Channels, Tandem Pore Domain
medicine
Bacteroides
Humans
Genetic Predisposition to Disease
International HapMap Project
General Dentistry
Genotyping
Genetics
Fusobacterium nucleatum
F-Box Proteins
Interleukins
Calcium-Binding Proteins
Human microbiome
Nucleic Acid Hybridization
Zinc Fingers
Middle Aged
medicine.disease
biology.organism_classification
Interleukin-33
Chronic periodontitis
Bacterial Load
Clinical Trials and Human Investigations
DNA-Binding Proteins
Repressor Proteins
Campylobacter rectus
Chronic Periodontitis
Trans-Activators
Metagenome
Female
Prevotella nigrescens
Porphyromonas gingivalis
Treponema denticola
Imputation (genetics)
Genome-Wide Association Study
Transcription Factors
Subjects
Details
- ISSN :
- 15440591
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Journal of dental research
- Accession number :
- edsair.doi.dedup.....08f518023ca81a3f0a113692a55c2054