Back to Search
Start Over
Comprehensive Analysis of Serum microRNAs in Autoimmune Pancreatitis
- Source :
- Digestion. 91:263-271
- Publication Year :
- 2015
- Publisher :
- S. Karger AG, 2015.
-
Abstract
- Background/Aims: Autoimmune pancreatitis (AIP) is a rare disease that has recently emerged as a unique type of pancreatitis with a presumed autoimmune etiology. MicroRNA (miRNA) is a small non-coding RNA that targets multiple mRNAs. miRNAs might exist in serum in a stabilized form, suggesting its potential application as a biomarker. We here examined the miRNA expression profile in the serum of patients with AIP. Methods: miRNAs were prepared from serum samples of patients with various pancreatic diseases (AIP (n = 3, each before and after the steroid therapy), chronic pancreatitis (n = 5), pancreatic cancer (n = 5)) or healthy controls (n = 5). A human miRNA Oligo chip containing approximately 2,000 miRNAs was used to identify differentially expressed miRNAs. Ingenuity Pathway Analysis (IPA) was used for the integrated analysis of altered miRNAs. Results: Microarray analysis identified miRNAs highly expressed in the serum of patients with AIP: 13 miRNAs vs. CP, 204 miRNAs vs. pancreatic cancer, and 19 miRNAs vs. healthy controls. miR-150-5p was commonly upregulated in AIP compared to the other samples. IPA revealed the most biological processes affected by the steroid therapy including cellular development, cellular growth, and cell movement. Conclusion: Our results identified that miRNAs were differentially expressed in the serum of AIP patients.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Autoimmune Diseases
Pancreatic cancer
microRNA
medicine
Humans
Aged
Oligonucleotide Array Sequence Analysis
Autoimmune pancreatitis
business.industry
Gene Expression Profiling
Gastroenterology
Middle Aged
medicine.disease
Pancreatic Neoplasms
MicroRNAs
Pancreatitis
Immunology
Etiology
Biomarker (medicine)
Female
IgG4-related disease
business
Biomarkers
Rare disease
Subjects
Details
- ISSN :
- 14219867 and 00122823
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Digestion
- Accession number :
- edsair.doi.dedup.....08f5778192fa5d195639beb44de59e97