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Toxic effect of NiCl2 on development of the bursa of Fabricius in broiler chickens
- Source :
- Oncotarget
- Publication Year :
- 2015
- Publisher :
- Impact Journals LLC, 2015.
-
Abstract
- This study was conducted with objective of evaluating the toxic effects of nickel chloride (NiCl2) on development of bursa of Fabricius in broilers fed on diets supplemented with 0, 300, 600 and 900 mg/kg of NiCl2 for 42 days by using the methods of experimental pathology, flow cytometry (FCM), and quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that dietary NiCl2 in 300 mg/kg and over induced toxic suppression in the bursal development, which was characterized by decreasing lymphocytes histopathologically and relative weight, increasing G0/G1 phase (a prolonged nondividing state), reducing S phase (DNA replication) and proliferating index, and increasing percentages of apoptotic cells. Concurrently, the mRNA expression levels of bax, cytochrome c (cyt c), apoptotic peptidase activating factor 1 (Apaf-1), caspase-3, caspase-6, caspase-7 and caspase-9 were increased and the bcl-2 mRNA expression levels were decreased. The toxic suppression of bursal development finally impaired humoral immunity duo to the reduction of B lymphocyte population and B lymphocyte activity in the broiler chicken. This study provides new evidences for further studying the effect mechanism of Ni and Ni compoundson B-cell or bursa of Fabricius.
- Subjects :
- 0301 basic medicine
animal structures
Lymphocyte
Population
Apoptosis
NiCl2
lesion
Avian Proteins
03 medical and health sciences
0302 clinical medicine
Bcl-2-associated X protein
Immune system
Bursa of Fabricius
relative weight
Nickel
medicine
Animals
Lymphocyte Count
Immune response
education
Cell Proliferation
bcl-2-Associated X Protein
education.field_of_study
B-Lymphocytes
biology
Reverse Transcriptase Polymerase Chain Reaction
Cytochrome c
Body Weight
Cell Cycle
Broiler
Research Paper: Immunology
Immunity
Cytochromes c
Gene Expression Regulation, Developmental
Organ Size
Molecular biology
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Caspases
Immunology
biology.protein
Immunology and Microbiology Section
Apoptosis Regulatory Proteins
Chickens
Subjects
Details
- Language :
- English
- ISSN :
- 19492553
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....0906a21849dd729385befe3a31a3f274