Back to Search
Start Over
Sulfur dioxide induces vascular relaxation through PI3K/Akt/eNOS and NO/cGMP signaling pathways in rats
- Source :
- Human & Experimental Toxicology. 39:1108-1117
- Publication Year :
- 2020
- Publisher :
- SAGE Publications, 2020.
-
Abstract
- Sulfur dioxide (SO2) is a common exogenous atmospheric pollutant. Studies have shown that SO2can cause vasodilation as a gas signaling molecule, but the specific signaling pathways are not well understood. This study aimed to explore the underlying mechanism behind the effects of SO2on vasodilation of isolated rat aorta. The results showed that when the dose of SO2was 30 μM, the vasodilation of endothelium-intact rings was partially suppressed by LY294002 and NG-nitro-l-arginine methyl ester, and the protein levels of phosphoinositide 3-kinase (PI3K), p-Akt, and p-endothelial nitric oxide synthase ( p-eNOS) were significantly increased. When the dose of SO2was 300 μM or 1500 μM, the vasodilation of endothelium-denuded rings did not change after application of the inhibitor, but the protein levels of PI3K, p-Akt, and p-eNOS were significantly decreased, and the activity of NOS and the level of nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) were significantly increased. We speculate that the mechanism of SO2-induced vasodilatation likely involved the endothelial PI3K/Akt/eNOS and NO/cGMP signal pathways. In addition, at the concentration of 1500 μM, SO2markedly increased the level of caspase-3 and caspase-9. The results suggest that high concentrations of SO2may cause damage to blood vessels. This study will help to further inform the etiologies of SO2-related cardiovascular disease.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Nitric Oxide Synthase Type III
Health, Toxicology and Mutagenesis
Aorta, Thoracic
Vasodilation
030204 cardiovascular system & hematology
Nitric Oxide
Toxicology
Nitric oxide
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Enos
Internal medicine
Cyclic AMP
medicine
Animals
Sulfur Dioxide
LY294002
Cyclic GMP
Protein kinase B
Cyclic guanosine monophosphate
PI3K/AKT/mTOR pathway
Air Pollutants
biology
Chemistry
General Medicine
biology.organism_classification
Nitric oxide synthase
030104 developmental biology
Endocrinology
biology.protein
Phosphatidylinositol 3-Kinase
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 14770903 and 09603271
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Human & Experimental Toxicology
- Accession number :
- edsair.doi.dedup.....09135ac89c74b09eed989f772b495e99