Back to Search
Start Over
Long noncoding RNA lnc-ABCA12-3 promotes cell migration, invasion, and proliferation by regulating fibronectin 1 in esophageal squamous cell carcinoma
- Source :
- Journal of cellular biochemistry. 121(2)
- Publication Year :
- 2019
-
Abstract
- Long noncoding RNAs (lncRNAs) have been shown to play important roles in human cancers, including esophageal squamous cell carcinoma (ESCC). We previously demonstrated that a novel lncRNA, lnc-ABCA12-3, was overexpressed in ESCC tissues. However, the exact function of lnc-ABCA12-3 is unknown. In the current study, we aimed to evaluate the expression of lnc-ABCA12-3 in ESCC and to explore the potential mechanism of lnc-ABCA12-3 in cell migration, invasion, and proliferation. We showed that lnc-ABCA12-3 was upregulated in ESCC tumor tissues and cell lines. The increased expression of lnc-ABCA12-3 was positively associated with advanced tumor-node-metastasis stages and poor prognosis. The knockdown of lnc-ABCA12-3 inhibited the cell migration, invasion, and proliferation abilities of KYSE-510 and Eca-109 cells. We also found that fibronectin 1 (FN1) was upregulated in ESCC tumor tissues. The expression of FN1 messenger RNA was positively correlated with the expression of lnc-ABCA12-3 in ESCC tumor tissues. After lnc-ABCA12-3 knockdown, the expression of FN1 was downregulated. In addition, the overexpression of FN1 restored the abilities of cell migration, invasion and proliferation in Eca-109 cells. Further studies indicated that lnc-ABCA12-3 acted as a competing endogenous RNA for miR-200b-3p to regulate FN1 expression. In conclusion, these results suggest that lnc-ABCA12-3 is a novel oncogene in tumorigenesis and that its high expression is related to a poor prognosis for patients with ESCC. lnc-ABCA12-3 promotes cell migration, invasion, and proliferation via the regulation of FN1 in ESCC. Our data suggest that lnc-ABCA12-3 might serve as a potential prognostic biomarker and therapeutic target for ESCC.
- Subjects :
- 0301 basic medicine
Male
Esophageal Neoplasms
Apoptosis
medicine.disease_cause
Biochemistry
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Cell Movement
medicine
Biomarkers, Tumor
Tumor Cells, Cultured
Humans
Neoplasm Invasiveness
Molecular Biology
Cell Proliferation
Gene knockdown
Oncogene
biology
Competing endogenous RNA
Cell migration
Cell Biology
Middle Aged
Prognosis
digestive system diseases
Long non-coding RNA
Fibronectins
Fibronectin
Gene Expression Regulation, Neoplastic
Survival Rate
MicroRNAs
030104 developmental biology
030220 oncology & carcinogenesis
Cancer research
biology.protein
Female
RNA, Long Noncoding
Esophageal Squamous Cell Carcinoma
Carcinogenesis
Subjects
Details
- ISSN :
- 10974644
- Volume :
- 121
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of cellular biochemistry
- Accession number :
- edsair.doi.dedup.....0914a490a3dcb87682808fec29766302