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Evaluation of the Limiting Antigen Avidity EIA (LAg) among people who inject drugs in Greece
- Source :
- Epidemiology and infection
- Publication Year :
- 2016
-
Abstract
- SUMMARYThis analysis assessed the utility of the limiting antigen avidity assay (LAg). Samples of people who inject drugs (PWID) in Greece with documented duration of HIV-1 infection were tested by LAg. A LAg-normalized optical density (ODn) ⩽1·5 corresponds to a recency window period of 130 days. The proportion true recent (PTR) and proportion false recent (PFR) were estimated in 28 seroconverters and in 366 samples collected >6 months after HIV diagnosis, respectively. The association between LAg ODn and HIV RNA level was evaluated in 232 persons. The PTR was 85·7%. The PFR was 20·8% but fell to 5·9% in samples from treatment-naive individuals with long-standing infection (>1 year), and to 0 in samples with the circulating recombinant form CRF35 AD. A LAg-based algorithm with a PFR of 3·3% estimated a similar incidence trend to that calculated by analyses based on HIV-1 seroconversions. In recently infected persons indicated by LAg, the median log10HIV RNA level was high (5·30, interquartile range 4·56–5·90). LAg can help identify highly infectious HIV(+) individuals as it accurately identifies recent infections and is correlated with the HIV RNA level. It can also produce reliable estimates of HIV-1 incidence.
- Subjects :
- 0301 basic medicine
Male
Epidemiology
Limiting antigen avidity assay
Antibody Affinity
HIV Infections
Window period
Antiviral therapy
HIV Antibodies
Procedures
Evaluation study
Immunoenzyme Techniques
0302 clinical medicine
Interquartile range
Cd4 lymphocyte count
Hiv infections
Hiv antibodies
Viral load
030212 general & internal medicine
Viral
Substance Abuse, Intravenous
Immunoassay
medicine.diagnostic_test
Greece
Incidence (epidemiology)
Incidence
Immunoenzyme techniques
Virus rna
Viral Load
Diagnostic error
Infectious Diseases
Blood
Sensitivity and specificity
Seroconversion
RNA, Viral
Female
Intravenous
Human
Substance abuse
Adult
Drug injectors
Major clinical study
Drug abuse
Enzyme immunoassay
Article
03 medical and health sciences
Disease association
Antigen
medicine
Humans
Avidity
Diagnostic Errors
Disease duration
Diagnostic errors
business.industry
Hiv
Human immunodeficiency virus 1 infection
Virology
Lag
Rna analysis
030104 developmental biology
Human immunodeficiency virus antibody
Immunology
Rna
Virus load
Antibody affinity
Comparative study
Recent infection
business
Controlled study
Complication
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Epidemiology and infection
- Accession number :
- edsair.doi.dedup.....093a623a45cc73abee26c7b30b8cb73f