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Enhanced Antifungal Activity of Engineered Proteins via Swapping between Thioredoxin H2 and H3
- Source :
- Applied Sciences, Volume 9, Issue 22, Applied Sciences, Vol 9, Iss 22, p 4766 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- Thioredoxins (Trxs) are proteins that act as antioxidants by facilitating the reduction of other proteins and are highly conserved in all organisms. Plant H-type Trx isoforms have different structures and perform multiple functions. Previous studies have reported that the low molecular weight AtTrx-H2 acts as a disulfide reductase and the high molecular weight AtTrx-H3 functions as an oxidoreductase and a molecular chaperone. In this study, we compared the antifungal activities of Arabidopsis Trx-H2 and -H3 with engineered proteins 2N3C and 3N2C via domain-swapping between the N- and C-terminal regions of Trx-H2 and -H3. All AtTrx-H variant proteins inhibited cell growth of various pathogenic fungal strains at pH 5.2 and pH 7.2 and showed significant intracellular accumulation in the fungal cells. Interestingly, only two engineered proteins penetrated the fungal cell wall and membrane, indicating their ability to destabilize the fungal cell membrane before internalization into the cytosol. To our knowledge, this is the first study that demonstrates novel functions of plant antioxidants AtTrx-H2 and -H3 as antifungal proteins and shows their enhanced activity using the domain swapping technique.
- Subjects :
- 0301 basic medicine
swapping
lcsh:Technology
lcsh:Chemistry
Cell membrane
Cell wall
03 medical and health sciences
0302 clinical medicine
Oxidoreductase
Arabidopsis
medicine
General Materials Science
Thioredoxin
lcsh:QH301-705.5
Instrumentation
Fluid Flow and Transfer Processes
chemistry.chemical_classification
biology
lcsh:T
Cell growth
Chemistry
Process Chemistry and Technology
Arabidopsis thaliana
General Engineering
biology.organism_classification
lcsh:QC1-999
Computer Science Applications
Cytosol
030104 developmental biology
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
Biochemistry
lcsh:TA1-2040
030220 oncology & carcinogenesis
lcsh:Engineering (General). Civil engineering (General)
lcsh:Physics
Intracellular
antifungal
Subjects
Details
- Language :
- English
- ISSN :
- 20763417
- Database :
- OpenAIRE
- Journal :
- Applied Sciences
- Accession number :
- edsair.doi.dedup.....093cdc37ddba37fc32c56ce7f38b9af2
- Full Text :
- https://doi.org/10.3390/app9224766