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Circulating Cell-Free DNA Level in Prediction of COVID-19 Severity and Mortality: Correlation of with Haematology and Serum Biochemical Parameters

Authors :
Sridhar Mishra
Devanshi B. Dubey
Krachi Agarwal
Deval B. Dubey
Shweta Verma
Nida Shabbir
Rashmi Kushwaha
D Himanshu Reddy
Uma Shankar Singh
Wahid Ali
Source :
Indian Journal of Clinical Biochemistry. 38:172-181
Publication Year :
2022
Publisher :
Springer Science and Business Media LLC, 2022.

Abstract

Lymphocyte dysregulation in coronavirus disease-19 (COVID-19) is a major contributing factor linked to disease severity and mortality. Apoptosis results in the accumulation of cell-free DNA (cfDNA) in circulation. COVID-19 has a heterogeneous clinical course. The role of cfDNA levels was studied to assess the severity and outcome of COVID-19 patients and correlated with other laboratory parameters. The current case series included 100 patients with mild COVID-19 (MCOV-19) and 106 patients with severe COVID-19 (SCOV-19). Plasma cfDNA levels were quantified using SYBR green quantitative real-time PCR through amplification of the β-actin gene. CfDNA level was significantly higher in SCOV-19 at 706.7 ng/ml (522.6-1258) as compared to MCOV-19 at 219.8 ng/ml (167.7-299.6). The cfDNA levels were significantly higher in non-survivor than in survivors (

Subjects

Subjects :
Clinical Biochemistry

Details

ISSN :
09740422 and 09701915
Volume :
38
Database :
OpenAIRE
Journal :
Indian Journal of Clinical Biochemistry
Accession number :
edsair.doi.dedup.....0940f29c8735ead34b53c007c0f25cd8
Full Text :
https://doi.org/10.1007/s12291-022-01082-4