Back to Search
Start Over
The Perennial Ryegrass GenomeZipper – Targeted Use of Genome Resources for Comparative Grass Genomics
- Source :
- Pfeiffer, M, Martis, M, Asp, T, Mayer, K F X, Lübberstedt, T, Byrne, S, Frei, U & Studer, B 2013, ' The Perennial Ryegrass GenomeZipper – Targeted Use of Genome Resources for Comparative Grass Genomics ', Plant Physiology, vol. 161, no. 2, pp. 571-582 . https://doi.org/10.1104/pp.112.207282, Plant Physiol. 161, 571-582 (2013), PLANT PHYSIOLOGY, Pfeifer, M, Martis, M, Asp, T, Mayer, K F X, Lübberstedt, T, Byrne, S, Frei, U & Studer, B 2013, The Perennial Ryegrass GenomeZipper – Targeted Use of Genome Resources for Comparative Grass Genomics . in Plant Biotech Denmark Annual meeting 2013 . pp. 118, Plant Biotech Denmark Annual Meeting 2013, Copenhagen, Denmark, 31/01/2013 . < http://www.plant-biotech.dk/Annual_meeting.aspx >
- Publication Year :
- 2013
-
Abstract
- Whole-genome sequences established for model and major crop species constitute a key resource for advanced genomic research. For outbreeding forage and turf grass species like ryegrasses (Lolium spp.), such resources have yet to be developed. Here, we present a model of the perennial ryegrass (Lolium perenne) genome on the basis of conserved synteny to barley (Hordeum vulgare) and the model grass genome Brachypodium (Brachypodium distachyon) as well as rice (Oryza sativa) and sorghum (Sorghum bicolor). A transcriptome-based genetic linkage map of perennial ryegrass served as a scaffold to establish the chromosomal arrangement of syntenic genes from model grass species. This scaffold revealed a high degree of synteny and macrocollinearity and was then utilized to anchor a collection of perennial ryegrass genes in silico to their predicted genome positions. This resulted in the unambiguous assignment of 3,315 out of 8,876 previously unmapped genes to the respective chromosomes. In total, the GenomeZipper incorporates 4,035 conserved grass gene loci, which were used for the first genome-wide sequence divergence analysis between perennial ryegrass, barley, Brachypodium, rice, and sorghum. The perennial ryegrass GenomeZipper is an ordered, information-rich genome scaffold, facilitating map-based cloning and genome assembly in perennial ryegrass and closely related Poaceae species. It also represents a milestone in describing synteny between perennial ryegrass and fully sequenced model grass genomes, thereby increasing our understanding of genome organization and evolution in the most important temperate forage and turf grass species.
- Subjects :
- 0106 biological sciences
Physiology
Plant Science
Poaceae
01 natural sciences
Lolium perenne
Genome
Synteny
Chromosomes, Plant
03 medical and health sciences
Botany
Genetics
Lolium
Sorghum
030304 developmental biology
2. Zero hunger
0303 health sciences
Oryza sativa
biology
fungi
Chromosome Mapping
Computational Biology
Reproducibility of Results
food and beverages
Oryza
Genomics
15. Life on land
Breakthrough Technologies
biology.organism_classification
Agronomy
Brachypodium
Hordeum vulgare
Brachypodium distachyon
Transcriptome
Genome, Plant
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Pfeiffer, M, Martis, M, Asp, T, Mayer, K F X, Lübberstedt, T, Byrne, S, Frei, U & Studer, B 2013, ' The Perennial Ryegrass GenomeZipper – Targeted Use of Genome Resources for Comparative Grass Genomics ', Plant Physiology, vol. 161, no. 2, pp. 571-582 . https://doi.org/10.1104/pp.112.207282, Plant Physiol. 161, 571-582 (2013), PLANT PHYSIOLOGY, Pfeifer, M, Martis, M, Asp, T, Mayer, K F X, Lübberstedt, T, Byrne, S, Frei, U & Studer, B 2013, The Perennial Ryegrass GenomeZipper – Targeted Use of Genome Resources for Comparative Grass Genomics . in Plant Biotech Denmark Annual meeting 2013 . pp. 118, Plant Biotech Denmark Annual Meeting 2013, Copenhagen, Denmark, 31/01/2013 . < http://www.plant-biotech.dk/Annual_meeting.aspx >
- Accession number :
- edsair.doi.dedup.....094c24b5827e728e7b24cd57ec5c88c2
- Full Text :
- https://doi.org/10.1104/pp.112.207282