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Expression of biologically active heterodimeric bovine follicle-stimulating hormone in milk of transgenic mice
- Source :
- Proceedings of the National Academy of Sciences. 88:8327-8331
- Publication Year :
- 1991
- Publisher :
- Proceedings of the National Academy of Sciences, 1991.
-
Abstract
- Follicle-stimulating hormone (FSH; follitropin) is a pituitary glycoprotein composed of two post-translationally modified subunits, which must properly assemble to be biologically active. FSH has been difficult to purify and to obtain in quantities sufficient for detailed biochemical studies. We have targeted FSH expression to the mammary gland of transgenic mice by using cDNAs encoding the bovine alpha and FSH beta subunits and a modified rat beta-casein gene-based expression system. Lines of bigenic mice expressing both subunits have been generated either by coinjection of the subunit transgenes or by mating mice that acquired and expressed transgenes encoding an individual subunit. Up to 60 international units (15 micrograms) of biologically active FSH per ml was detected in the milk of the bigenic mice. These lines provide a model system for studying the post-transcriptional mechanisms that effect the expression and secretion of this heterodimeric hormone.
- Subjects :
- Genetically modified mouse
endocrine system
Transgene
Protein subunit
Genetic Vectors
Molecular Sequence Data
Oligonucleotides
Gene Expression
Mice, Transgenic
Follitropin
Biology
Polymerase Chain Reaction
Mice
Follicle-stimulating hormone
Mammary Glands, Animal
Gene expression
Animals
Lactation
Secretion
RNA, Messenger
Cloning, Molecular
Multidisciplinary
Base Sequence
Caseins
DNA
Blotting, Northern
Molecular biology
Blotting, Southern
Milk
Cattle
Follicle Stimulating Hormone
Research Article
Hormone
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 88
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....0954e154b1ec88126e330f39360a8053