Back to Search
Start Over
Effects of Salmonella infection on hepatic damage following acute liver injury in rats
- Source :
- Hepatobiliary & Pancreatic Diseases International. 15:399-405
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Background Acute liver injury is a common clinical disorder associated with intestinal barrier injury and disturbance of intestinal microbiota. Probiotic supplementation has been reported to reduce liver injury; however, it is unclear whether enteropathogen infection exacerbates liver injury. The purpose of this study was to address this unanswered question using a rat model. Methods Oral supplementation with Salmonella enterica serovar enteritidis ( S. enteritidis ) was given to rats for 7 days. Different degrees of acute liver injury were then induced by intraperitoneal injection of D-galactosamine. The presence and extent of liver injury was assayed by measuring the concentrations of serum alanine aminotransferase, aspartate aminotransferase, and total bilirubin. Histology was used to observe liver tissue damage. Additionally, we measured the changes in plasma endotoxin, serum cytokines and bacterial translocation to clarify the mechanisms underlying intestinal microbiota associated liver injury. Results The levels of liver damage and endotoxin were significantly increased in the Salmonella infected rats with severe liver injury compared with the no infection rats with severe liver injury ( P >0.01); The peyer's patch CD3 + T cell counts were increased significantly when the Salmonella infection with severe injury group was compared with the normal group ( P >0.05). S. enteritidis pretreatment enhanced intestinal barrier impairment and bacterial translocation. Conclusions Oral S. enteritidis administration exacerbates acute liver injury, especially when injury was severe. Major factors of the exacerbation include inflammatory and oxidative stress injuries induced by the translocated bacteria and associated endotoxins, as well as over-activation of the immune system in the intestine and liver.
- Subjects :
- Male
0301 basic medicine
Time Factors
CD3 Complex
Bilirubin
T-Lymphocytes
Salmonella enteritidis
medicine.medical_treatment
Intraperitoneal injection
Galactosamine
Salmonella infection
medicine.disease_cause
Rats, Sprague-Dawley
Peyer's Patches
03 medical and health sciences
chemistry.chemical_compound
Immune system
medicine
Animals
Aspartate Aminotransferases
Liver injury
Hepatology
biology
business.industry
Gastroenterology
Alanine Transaminase
medicine.disease
Endotoxins
Disease Models, Animal
030104 developmental biology
Liver
chemistry
Alanine transaminase
Bacterial Translocation
Acute Disease
Host-Pathogen Interactions
Salmonella Infections
Immunology
biology.protein
Cytokines
Chemical and Drug Induced Liver Injury
business
Biomarkers
Oxidative stress
Subjects
Details
- ISSN :
- 14993872
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Hepatobiliary & Pancreatic Diseases International
- Accession number :
- edsair.doi.dedup.....095bc142804f539838c5d12bafb99eb6