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Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin
- Source :
- International Journal of Biological Sciences
- Publication Year :
- 2014
- Publisher :
- Ivyspring International Publisher, 2014.
-
Abstract
- Catalpol and puerarin are active ingredients isolated from Rehmannia glutinosa Libosch and Radix Puerariae, respectively. They are popular in research for their poly-pharmacological effects. This research focused on effect of anti-stroke by lyophilized powder of catalpol and puerarin (C-P) and potential mechanisms. At the beginning of research, C-P was identified and analyzed by HPLC. Neurological function was evaluated by Longa score, neurological complex function score and beam balance score after permanent middle cerebral artery occlusion (PMCAO) in mice. Infarct volume and water content were evaluated after treatment of C-P. Anti-oxidative stress, anti-apoptosis, angiogenesis and neurogenesis were investigated by ELISA, WB and immunohistochemical stain respectively. With treatment of C-P, neurological deficiency of PMCAO mice was ameliorated. Morphologically, infarct volume and water content in ischemic hemisphere were significantly reduced by C-P. In vivo and in vitro, oxidative stress injury was extenuated by C-P. Meanwhile, Caspase-3 was down-regulated and Bxl-2 was up-regulated by C-P in vivo. In addition, C-P enhanced angiogenesis around the infarct of cortex and neurogenesis in the Hippocampal Dentate Gyrus (DG). Hence, C-P ameliorated stroke-induced neurological deficiency through its multiple neuroprotections. What's more, this article provides us a novel formula of active ingredients for stroke.
- Subjects :
- Male
Neurogenesis
catapol
Neovascularization, Physiologic
Apoptosis
Pharmacology
medicine.disease_cause
PC12 Cells
Applied Microbiology and Biotechnology
Mice
chemistry.chemical_compound
Puerarin
In vivo
Animals
Medicine
Molecular Biology
Stroke
Ecology, Evolution, Behavior and Systematics
biology
business.industry
neurological function
Dentate gyrus
puerarin
Cell Biology
medicine.disease
Rehmannia glutinosa
biology.organism_classification
stroke
Immunohistochemistry
Isoflavones
Catalpol
Rats
Quaternary Ammonium Compounds
Oxidative Stress
Freeze Drying
chemistry
Immunology
Powders
business
Oxidative stress
Research Paper
Developmental Biology
Subjects
Details
- ISSN :
- 14492288
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....0961c8997f09b5e102851cc7cf8bfe48
- Full Text :
- https://doi.org/10.7150/ijbs.8571