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Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using Punica granatum Leaves
- Source :
- Molecules, Volume 26, Issue 19, Molecules, Vol 26, Iss 5762, p 5762 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- The current research work illustrates an economical and rapid approach towards the biogenic synthesis of silver nanoparticles using aqueous Punica granatum leaves extract (PGL-AgNPs). The optimization of major parameters involved in the biosynthesis process was done using Box-Behnken Design (BBD). The effects of different independent variables (parameters), namely concentration of AgNO3, temperature and ratio of extract to AgNO3, on response viz. particle size and polydispersity index were analyzed. As a result of experiment designing, 17 reactions were generated, which were further validated experimentally. The statistical and mathematical approaches were employed on these reactions in order to interpret the relationship between the factors and responses. The biosynthesized nanoparticles were initially characterized by UV-vis spectrophotometry followed by physicochemical analysis for determination of particle size, polydispersity index and zeta potential via dynamic light scattering (DLS), SEM and EDX studies. Moreover, the determination of the functional group present in the leaves extract and PGL-AgNPs was done by FTIR. Antibacterial and antibiofilm efficacies of PGL-AgNPs against Gram-positive and Gram-negative bacteria were further determined. The physicochemical studies suggested that PGL-AgNPs were round in shape and of ~37.5 nm in size with uniform distribution. Our studies suggested that PGL-AgNPs exhibit potent antibacterial and antibiofilm properties.
- Subjects :
- silver nanoparticles
Dispersity
Metal Nanoparticles
Pharmaceutical Science
Nanoparticle
Microbial Sensitivity Tests
Gram-Positive Bacteria
Article
Pomegranate
Silver nanoparticle
Analytical Chemistry
QD241-441
Dynamic light scattering
Gram-Negative Bacteria
Drug Discovery
Zeta potential
Punica granatum leaves extract
Particle Size
Physical and Theoretical Chemistry
nanotechnology
biology
Plant Extracts
Chemistry
antibiofilm
Organic Chemistry
biology.organism_classification
Box–Behnken design
Anti-Bacterial Agents
Plant Leaves
antibacterial
Chemistry (miscellaneous)
Biofilms
Punica
Silver Nitrate
Molecular Medicine
Particle size
Box-Behnken design
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....0987c05e57aa2f0366219e5163678754
- Full Text :
- https://doi.org/10.3390/molecules26195762