Back to Search
Start Over
Fluorescent nanodiamonds as a robust temperature sensor inside a single cell
- Source :
- Biophysics and Physicobiology
- Publication Year :
- 2018
- Publisher :
- The Biophysical Society of Japan (BSJ), 2018.
-
Abstract
- Thermometers play an important role to study the biological significance of temperature. Fluorescent nanodiamonds (FNDs) with negatively-charged nitrogen-vacancy centers, a novel type of fluorescence-based temperature sensor, have physicochemical inertness, low cytotoxicity, extremely stable fluorescence, and unique magneto-optical properties that allow us to measure the temperature at the nanoscale level inside single cells. Here, we demonstrate that the thermosensing ability of FNDs is hardly influenced by environmental factors, such as pH, ion concentration, viscosity, molecular interaction, and organic solvent. This robustness renders FNDs reliable thermometers even under complex biological cellular environment. Moreover, the simple protocol developed here for measuring the absolute temperature inside a single cell using a single FND enables successful temperature measurement in a cell with an accuracy better than ±1°C.
- Subjects :
- 0301 basic medicine
Materials science
Temperature sensing
Cellular imaging
Organic solvent
Nanotechnology
Regular Article
General Medicine
nitrogen-vacancy center
Fluorescence
Temperature measurement
magnetic resonance
03 medical and health sciences
030104 developmental biology
Biological significance
cellular imaging
fluorescent nanodiamond
Nitrogen-vacancy center
Nanoscopic scale
temperature sensing
Subjects
Details
- Language :
- English
- ISSN :
- 21894779
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Biophysics and Physicobiology
- Accession number :
- edsair.doi.dedup.....09a501871a1bf6eac373355431190968