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ArcR contributes to tolerance to fluoroquinolone antibiotics by regulating katA in Staphylococcus aureus

Authors :
Fu, Tongtong
Fan, Zheng
Li, Yujie
Li, Zhoufei
Du, Bing
Liu, Shiyu
Cui, Xiaohu
Zhang, Rui
Zhao, Hanqing
Feng, Yanling
Xue, Guanhua
Cui, Jinghua
Yan, Chao
Gan, Lin
Feng, Junxia
Xu, Ziying
Yu, Zihui
Tian, Ziyan
Ding, Zanbo
Chen, Jinfeng
Chen, Yujie
Yuan, Jing
Source :
Frontiers in Microbiology. 14
Publication Year :
2023
Publisher :
Frontiers Media SA, 2023.

Abstract

Staphylococcus aureus is an opportunistic pathogen that shows a unique ability to quickly respond to a variety of antibiotics. The Crp/Fnr family transcriptional regulator ArcR controls expression of arginine deiminase pathway genes arcABDC, which enable the utilization of arginine as an energy source for cell growth under anaerobic conditions. However, ArcR shares low overall similarity with other Crp/Fnr family proteins, suggesting that they differ in the response to environmental stress. In this study, MIC and survival assays were performed to determine the role of ArcR in antibiotic resistance and tolerance. The results showed that deletion of arcR reduced tolerance of S.aureus to fluoroquinolone antibiotics, mainly through a defect in the response to oxidative stress. In ΔarcR mutant, the expression of the major catalase gene katA was downregulated, and katA overexpression restored bacterial resistance to oxidative stress and antibiotics. We showed that ArcR directly regulated katA transcription by binding to the promoter region of katA. Therefore, our results revealed the contribution of ArcR in bacterial tolerance to oxidative stress and subsequently to fluoroquinolones antibiotics. This study added our understanding on the role of Crp/Fnr family in bacterial susceptibility to antibiotics.

Details

ISSN :
1664302X
Volume :
14
Database :
OpenAIRE
Journal :
Frontiers in Microbiology
Accession number :
edsair.doi.dedup.....09b405baf39742ba55b8feb926b835e6