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Neutrophil activation and survival are modulated by interaction with NK cells
- Source :
- International immunology. 22(10)
- Publication Year :
- 2010
-
Abstract
- It is increasingly evident that neutrophils are able to cross-talk with other leukocytes to shape ongoing inflammatory and immune responses. In this study, we analyzed whether human NK cells may influence the survival and activation of neutrophils under co-culture conditions. We report that NK cells exposed to either IL-15 or IL-18 alone strongly protect the survival of neutrophils via the release of IFNγ and granulocyte macrophage colony-stimulating factor (GM-CSF) plus IFNγ, respectively, and cause a slight up-regulation of neutrophil CD64 and CD11b expression. In comparison, NK cells exposed to both IL-15 and IL-18 show a lesser ability to increase the survival of neutrophils but can more potently up-regulate CD64 and CD11b expression, as well as induce the de novo surface expression of CD69, in neutrophils. Analysis of the events occurring in neutrophil/NK co-cultures exposed to IL-15 plus IL-18 revealed that (i) neutrophil survival is positively affected by NK-derived GM-CSF but negatively influenced by a CD18-dependent neutrophil/NK contact, (ii) NK-derived IFNγ is almost entirely responsible for the induction of CD64, (iii) both soluble factors (primarily GM-CSF) and direct cell-cell contact up-regulate CD11b and CD69 and (iv) NK-derived GM-CSF induces the expression of biologically active heparin-binding EGF-like growth factor (HB-EGF) in neutrophils. Finally, we demonstrate that NK cells can also express HB-EGF when stimulated with either IL-2 or IL-15, yet independently of endogenous GM-CSF. Altogether, our results define a novel interaction within the innate immune system whereby NK cells, by directly modulating neutrophil functions, might contribute to the pathogenesis of inflammatory diseases.
- Subjects :
- Cell Survival
Neutrophils
Immunology
Inflammation
Apoptosis
Cell Communication
Biology
Granulocyte
Neutrophil Activation
Interleukin 21
medicine
Immunology and Allergy
Macrophage
Humans
Cells, Cultured
Innate immune system
General Medicine
apoptosis
cytokines
HB-EGF
inflammation
Coculture Techniques
Cell biology
Killer Cells, Natural
Granulocyte macrophage colony-stimulating factor
medicine.anatomical_structure
Gene Expression Regulation
Interleukin 15
Interleukin 12
Cytokines
Intercellular Signaling Peptides and Proteins
medicine.symptom
medicine.drug
Heparin-binding EGF-like Growth Factor
Subjects
Details
- ISSN :
- 14602377
- Volume :
- 22
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- International immunology
- Accession number :
- edsair.doi.dedup.....09fac988dcc406723b8deae6f72fe3b5