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Loss of the CBX7 gene expression correlates with a highly malignant phenotype in thyroid cancer
- Source :
- Cancer research (Chic. Ill.) 68 (2008): 6770–6778. doi:10.1158/0008-5472.CAN-08-0695, info:cnr-pdr/source/autori:Pierlorenzo Pallante,1,2 Antonella Federico,2 Maria Teresa Berlingieri,1 Mimma Bianco,1 Angelo Ferraro,2 Floriana Forzati,1 Antonino Iaccarino,3 Maria Russo,3 Giovanna Maria Pierantoni,1 Vincenza Leone,2 Silvana Sacchetti,2 Giancarlo Troncone,2,3 Massimo Santoro,1,2 and Alfredo Fusco1,2/titolo:Loss of the CBX7 gene expression correlates with a highly malignant phenotype in thyroid cancer./doi:10.1158%2F0008-5472.CAN-08-0695/rivista:Cancer research (Chic. Ill.)/anno:2008/pagina_da:6770/pagina_a:6778/intervallo_pagine:6770–6778/volume:68
- Publication Year :
- 2008
-
Abstract
- Using gene expression profiling, we found that the CBX7 gene was drastically down-regulated in six thyroid carcinoma cell lines versus control cells. The aims of this study were to determine whether CBX7 is related to the thyroid cancer phenotype and to try to identify new tools for the diagnosis and prognosis of thyroid cancer. We thus evaluated CBX7 expression in various snap-frozen and paraffin-embedded thyroid carcinoma tissues of different degrees of malignancy by quantitative reverse transcription-PCR and immunohistochemistry, respectively. CBX7 expression progressively decreased with malignancy grade and neoplasia stage. Indeed, it decreased in an increasing percentage of cases going from benign adenomas to papillary (PTC), follicular, and anaplastic (ATC) thyroid carcinomas. This finding coincides with results obtained in rat and mouse models of thyroid carcinogenesis. CBX7 loss of heterozygosity occurred in 36.8% of PTC and in 68.7% of ATC. Restoration of CBX7 expression in thyroid cancer cells reduced growth rate, with a retention in the G1 phase of the cell cycle, suggesting that CBX7 can contribute to the proliferation of the transformed thyroid cells. In conclusion, loss of CBX7 expression correlates with a highly malignant phenotype in thyroid cancer patients. [Cancer Res 2008;68(16):6770–8]
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
endocrine system
endocrine system diseases
carcinomas
Chromosomes, Human, Pair 22
Blotting, Western
Thyroid Gland
Loss of Heterozygosity
Mice, Nude
Biology
medicine.disease_cause
Malignancy
Adenoviridae
thyroid
Colony-Forming Units Assay
Loss of heterozygosity
Thyroid carcinoma
Mice
oncogene
Cell Line, Tumor
Adenocarcinoma, Follicular
medicine
Carcinoma
Animals
Humans
RNA, Messenger
RNA, Neoplasm
Thyroid Neoplasms
Thyroid cancer
Cyclin-Dependent Kinase Inhibitor p16
Cell Proliferation
Polycomb Repressive Complex 1
Reverse Transcriptase Polymerase Chain Reaction
Thyroid
cbx 7
medicine.disease
Carcinoma, Papillary
Rats
Gene Expression Regulation, Neoplastic
Repressor Proteins
medicine.anatomical_structure
Oncology
CBX7
Adenocarcinoma
Carcinogenesis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Cancer research (Chic. Ill.) 68 (2008): 6770–6778. doi:10.1158/0008-5472.CAN-08-0695, info:cnr-pdr/source/autori:Pierlorenzo Pallante,1,2 Antonella Federico,2 Maria Teresa Berlingieri,1 Mimma Bianco,1 Angelo Ferraro,2 Floriana Forzati,1 Antonino Iaccarino,3 Maria Russo,3 Giovanna Maria Pierantoni,1 Vincenza Leone,2 Silvana Sacchetti,2 Giancarlo Troncone,2,3 Massimo Santoro,1,2 and Alfredo Fusco1,2/titolo:Loss of the CBX7 gene expression correlates with a highly malignant phenotype in thyroid cancer./doi:10.1158%2F0008-5472.CAN-08-0695/rivista:Cancer research (Chic. Ill.)/anno:2008/pagina_da:6770/pagina_a:6778/intervallo_pagine:6770–6778/volume:68
- Accession number :
- edsair.doi.dedup.....0a10c26ef4c680ab88217f49e97a03f3