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Does sperm origin affect embryo morphokinetic parameters?

Authors :
Aurore Catteau
Arnaud Reignier
Laurent David
Thomas Fréour
Jenna Lammers
Paul Barrière
C. Splingart
Le Bihan, Sylvie
Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI)
Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE)
Université de Nantes (UN)-Université de Nantes (UN)
Service de Médecine et Biologie du Développement et de la Reproduction [CHU Nantes]
Centre hospitalier universitaire de Nantes (CHU Nantes)
Institut de transplantation urologie-néphrologie (ITUN)
Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)
Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE)
Université de Nantes (UN)
Structure fédérative de recherche François Bonamy (SFR François Bonamy)
Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Santé de l'Université de Nantes (IRS-UN)
Clínica Eugin [Barcelona, Spain]
Source :
Journal of Assisted Reproduction and Genetics, Journal of Assisted Reproduction and Genetics, 2015, 32 (9), pp.1325-1332. ⟨10.1007/s10815-015-0517-z⟩
Publication Year :
2015
Publisher :
Springer Science and Business Media LLC, 2015.

Abstract

International audience; Purpose The purpose of our study was to use time-lapse in order to evaluate the impact of sperm origin (fresh ejaculate or surgically retrieved) on embryo morphokinetic parameters and clinical outcome in intracytoplasmic sperm injection (ICSI) cycles.Methods This retrospective monocentric study was conducted in 485 unselected couples undergoing 604 ICSI cycles with embryo culture in the Embryoscope®. Among them, 445 couples underwent ICSI cycle with fresh ejaculated sperm and 40 with surgically retrieved sperm (26 with testicular sperm and 14 with epididymal sperm). Embryo morphokinetic parameters and clinical cycle outcome were compared between fresh ejaculated sperm and surgically retrieved sperm. A subgroup analysis was also conducted between testicular and epididymal sperm ICSI cycles.Results Clinical outcome was comparable between groups according to sperm origin. Although most early morphokinetic parameters were comparable between ejaculated and surgical sperm groups, a few parameters were significantly different between both groups, but with a considerable overlap in their distribution. Late cellular events occurred significantly later in the surgical sperm group than in the ejaculated sperm group. Conclusions Morphokinetic analysis did not allow us to identify clinically relevant differences between fresh ejaculate and surgically retrieved sperm groups. Further studies are needed, especially concerning the relationship between sperm origin and late morphokinetic parameters, such as blastocyst development.

Details

ISSN :
15737330 and 10580468
Volume :
32
Database :
OpenAIRE
Journal :
Journal of Assisted Reproduction and Genetics
Accession number :
edsair.doi.dedup.....0a12aee912c4f31cbfd8bc2a2116f6ce
Full Text :
https://doi.org/10.1007/s10815-015-0517-z