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Comparison of separation methods for immunomodulatoryextracellular vesicles from helminths
- Publication Year :
- 2022
- Publisher :
- Zenodo, 2022.
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Abstract
- Helminths survive within their host by secreting immunomodulatory compounds, which hold therapeutic potential for inflammatory conditions. Helminth-derived extracellular vesicles (EVs) are one such component proposed to possess immunomodulatory activities. Due to the recent discovery of helminth EVs, standardized protocols for EV separation are lacking. Excretory/secretory products of the porcine helminth, Ascaris suum, were used to compare three EV separation methods: Size exclusion chromatography (SEC), ultracentrifugation (UC) and a combination of the two.TheirperformancewasevaluatedbyEVyield, sample purity and the ability of EVs to suppress lipopolysaccharide (LPS)-induced inflammation in vitro. We found that all three separation methods successfully separated helminth EVs with a similar EV yield. Functional studies showed that EVs from all three methods reducedLPS-induced levels of tumour necrosis factor (TNF-α) in a dose-dependent manner. Overall, the three separation methods showed similar performance, however, the combination of UC+SEC presented with slightly higher purity than either alone.
- Subjects :
- parasitic diseases
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....0a15f7e5c990515d130d84692d0a9a5f
- Full Text :
- https://doi.org/10.5281/zenodo.6610789