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MALDI-MSI spatially maps N-glycan alterations to histologically distinct pulmonary pathologies following irradiation
- Source :
- Scientific Reports, Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group UK, 2020.
-
Abstract
- Radiation-induced lung injury is a highly complex combination of pathological alterations that develop over time and severity of disease development is dose-dependent. Following exposures to lethal doses of irradiation, morbidity and mortality can occur due to a combination of edema, pneumonitis and fibrosis. Protein glycosylation has essential roles in a plethora of biological and immunological processes. Alterations in glycosylation profiles have been detected in diseases ranging from infection, inflammation and cancer. We utilized mass spectrometry imaging to spatially map N-glycans to distinct pathological alterations during the clinically latent period and at 180 days post-exposure to irradiation. Results identified alterations in a number of high mannose, hybrid and complex N-glycans that were localized to regions of mucus and alveolar-bronchiolar hyperplasia, proliferations of type 2 epithelial cells, accumulations of macrophages, edema and fibrosis. The glycosylation profiles indicate most alterations occur prior to the onset of clinical symptoms as a result of pathological manifestations. Alterations in five N-glycans were identified as a function of time post-exposure. Understanding the functional roles N-glycans play in the development of these pathologies, particularly in the accumulation of macrophages and their phenotype, may lead to new therapeutic avenues for the treatment of radiation-induced lung injury.
- Subjects :
- 0301 basic medicine
Male
Pathology
medicine.medical_specialty
Glycosylation
Pulmonary Fibrosis
lcsh:Medicine
Inflammation
Lung injury
Article
Imaging
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Fibrosis
Polysaccharides
Edema
Macrophages, Alveolar
medicine
Animals
lcsh:Science
Radiation Injuries
Lung
Pneumonitis
Multidisciplinary
Mass spectrometry
business.industry
Macrophages
lcsh:R
Cancer
Lung Injury
Pneumonia
Hyperplasia
medicine.disease
Macaca mulatta
030104 developmental biology
Phenotype
chemistry
030220 oncology & carcinogenesis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
lcsh:Q
medicine.symptom
business
Mannose
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....0a2df052de92a68352dbfd1e71f1613e