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Dok-3 deficient mice display different immune clustering and Tim-3 expression
- Source :
- European Journal of Medical Research, European Journal of Medical Research, Vol 24, Iss 1, Pp 1-5 (2019)
- Publication Year :
- 2019
- Publisher :
- BioMed Central, 2019.
-
Abstract
- Background Dok-3 has been shown to play an important role in immune system. Tim-3 also has been recognized as an important immune regulator which involves in many diseases. The relationship of them is still unclear. Methods We detected the expression of Tim-3 on spleen immune cells from Dok-3 deficient mice and control mice by flow cytometry. Results In this article, we found that Dok-3−/− mice display almost entirely different immune clustering characteristics compared with wild type 129 mice. The CD4 T cells and CD8 T cells decreased and DC cells, macrophages, MDSCs increased when the Dok-3 gene knocked-out. The Tim-3 expression on CD4 T cells, CD8 T cells, NK cells, DC cells increased when the Dok-3 gene knocked-out. The macrophages and MDSCs just display the opposite results. Conclusions Although Dok-3−/− mice display different immune clustering and Tim-3 expression, the mechanism still needs further study. Electronic supplementary material The online version of this article (10.1186/s40001-019-0384-7) contains supplementary material, which is available to authorized users.
- Subjects :
- CD4-Positive T-Lymphocytes
Regulator
lcsh:Medicine
Spleen
Tim-3
030204 cardiovascular system & hematology
Biology
CD8-Positive T-Lymphocytes
Flow cytometry
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
Deficient mouse
medicine
Cytotoxic T cell
Immune cells proportion
Animals
030212 general & internal medicine
Gene
Hepatitis A Virus Cellular Receptor 2
Adaptor Proteins, Signal Transducing
medicine.diagnostic_test
Research
Macrophages
Myeloid-Derived Suppressor Cells
lcsh:R
Wild type
General Medicine
Dendritic Cells
Cell biology
Killer Cells, Natural
medicine.anatomical_structure
Dok-3
Subjects
Details
- Language :
- English
- ISSN :
- 2047783X and 09492321
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- European Journal of Medical Research
- Accession number :
- edsair.doi.dedup.....0a3b62820c778a2eb8fc49c2fa1053f9