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Characterization of Endothelial Progenitor Cell Interactions with Human Tropoelastin
- Source :
- PLoS ONE, PLoS ONE, Vol 10, Iss 6, p e0131101 (2015)
- Publication Year :
- 2015
- Publisher :
- Public Library of Science, 2015.
-
Abstract
- The deployment of endovascular implants such as stents in the treatment of cardiovascular disease damages the vascular endothelium, increasing the risk of thrombosis and promoting neointimal hyperplasia. The rapid restoration of a functional endothelium is known to reduce these complications. Circulating endothelial progenitor cells (EPCs) are increasingly recognized as important contributors to device re-endothelialization. Extracellular matrix proteins prominent in the vessel wall may enhance EPC-directed re-endothelialization. We examined attachment, spreading and proliferation on recombinant human tropoelastin (rhTE) and investigated the mechanism and site of interaction. EPCs attached and spread on rhTE in a dose dependent manner, reaching a maximal level of 56±3% and 54±3%, respectively. EPC proliferation on rhTE was comparable to vitronectin, fibronectin and collagen. EDTA, but not heparan sulfate or lactose, reduced EPC attachment by 81±3%, while full attachment was recovered after add-back of manganese, inferring a classical integrin-mediated interaction. Integrin αVβ3 blocking antibodies decreased EPC adhesion and spreading on rhTE by 39±3% and 56±10% respectively, demonstrating a large contribution from this specific integrin. Attachment of EPCs on N-terminal rhTE constructs N25 and N18 accounted for most of this interaction, accompanied by comparable spreading. In contrast, attachment and spreading on N10 was negligible. αVβ3 blocking antibodies reduced EPC spreading on both N25 and N18 by 45±4% and 42±14%, respectively. In conclusion, rhTE supports EPC binding via an integrin mechanism involving αVβ3. N25 and N18, but not N10 constructs of rhTE contribute to EPC binding. The regulation of EPC activity by rhTE may have implications for modulation of the vascular biocompatibility of endovascular implants.
- Subjects :
- Adult
Male
Endothelium
Integrin
lcsh:Medicine
02 engineering and technology
Endothelial progenitor cell
03 medical and health sciences
Young Adult
Tropoelastin
medicine
Cell Adhesion
Humans
Progenitor cell
lcsh:Science
Cells, Cultured
030304 developmental biology
Cell Proliferation
Endothelial Progenitor Cells
Neointimal hyperplasia
0303 health sciences
Multidisciplinary
biology
Tissue Scaffolds
Chemistry
lcsh:R
021001 nanoscience & nanotechnology
medicine.disease
Recombinant Proteins
3. Good health
Cell biology
Fibronectin
medicine.anatomical_structure
Immunology
embryonic structures
biology.protein
cardiovascular system
Vitronectin
lcsh:Q
Stents
Endothelium, Vascular
0210 nano-technology
circulatory and respiratory physiology
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....0a4018722d6565c7cabb98e6ec30fb06