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Axin-1 Regulates Meiotic Spindle Organization in Mouse Oocytes
- Source :
- PLoS ONE, Vol 11, Iss 6, p e0157197 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- Axin-1, a negative regulator of Wnt signaling, is a versatile scaffold protein involved in centrosome separation and spindle assembly in mitosis, but its function in mammalian oogenesis remains unknown. Here we examined the localization and function of Axin-1 during meiotic maturation in mouse oocytes. Immunofluorescence analysis showed that Axin-1 was localized around the spindle. Knockdown of the Axin1 gene by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in severely defective spindles, misaligned chromosomes, failure of first polar body (PB1) extrusion, and impaired pronuclear formation. However, supplementing the culture medium with the Wnt pathway activator LiCl improved spindle morphology and pronuclear formation. Downregulation of Axin1 gene expression also impaired the spindle pole localization of γ-tubulin/Nek9 and resulted in retention of the spindle assembly checkpoint protein BubR1 at kinetochores after 8.5 h of culture. Our results suggest that Axin-1 is critical for spindle organization and cell cycle progression during meiotic maturation in mouse oocytes.
- Subjects :
- 0301 basic medicine
Centrosomes
lcsh:Medicine
Cell Cycle Proteins
Immunostaining
Biochemistry
Spindle pole body
Mice
0302 clinical medicine
Oogenesis
Animal Cells
Tubulin
NIMA-Related Kinases
Small interfering RNAs
Cell Cycle and Cell Division
RNA, Small Interfering
lcsh:Science
Asses
Cells, Cultured
Staining
Mammals
Multidisciplinary
Meiotic spindle organization
Kinetochore
Chromosome Biology
Microinjection
Cell biology
Nucleic acids
Spindle checkpoint
Meiosis
Cell Processes
030220 oncology & carcinogenesis
OVA
Vertebrates
Female
RNA Interference
Centrosome separation
Cellular Types
Cellular Structures and Organelles
Research Article
Equines
Spindle Apparatus
macromolecular substances
Biology
Protein Serine-Threonine Kinases
Research and Analysis Methods
03 medical and health sciences
Axin Protein
Genetics
Animals
Non-coding RNA
Molecular Biology Techniques
Molecular Biology
lcsh:R
Organisms
Biology and Life Sciences
Cell Biology
Spindle apparatus
Gene regulation
030104 developmental biology
Germ Cells
Specimen Preparation and Treatment
Amniotes
Oocytes
Spindle organization
RNA
lcsh:Q
Gene expression
Multipolar spindles
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....0a4b04fa46ffadb16fc4a2a2cc7ea351