Supplementary Figures from Recurrent TRIO Fusion in Nontranslocation–Related Sarcomas

Supplementary Figure 1: Identification of FARSA-SYCE2 and GSE1-RP11-680G10.1 read-throughs in sarcoma CL1 and normal tissue. Supplementary Figure 2: aCGH (SNP-array) profile of CL1 which present a genomic rearrangement of TRIO and TERT genes. Supplementary Figure 3: Expression percentage of the two TRIO-TERT isoforms in three cases. Supplementary Figure 4: Predicted acceptor splicing regions in intron 2 and 3 of LINC01504. Supplementary Figure 5: Transcriptomic profile clustering of the 117 non-translocation-related sarcomas. Supplementary Figure 6: Volcano plot of the 117 sarcomas with six fused TRIO cases versus 111 non-fused TRIO cases. Supplementary Figure 7: Exonic expression of fused TRIO fused and non-fused TRIO cases.Supplementary Figure 8: Enrichment plot for significant Hallmarks in six fused TRIO samples included in the MSig5.0 database according to GSEA. Supplementary Figure 9: Enrichment plot for significant Hallmarks in 111 non-fused TRIO samples included in the MSig5.0 database according to GSEA. Supplementary Figure 10: ALT mechanism status determined by PML/TERF2 immunofluorescence analysis.