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Proteomic profile changes associated with diminished expression of T-cell intracellular antigens reveal a hormesis response
- Source :
- Biochemical and Biophysical Research Communications. 503:2569-2575
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- T-cell intracellular antigen (TIA) proteins function as regulators of cell homeostasis by controlling global gene expression in response to dynamic regulatory changes and environmental stress. Here, we used two-dimensional differential in-gel electrophoresis (2D-DIGE) and mass spectrometry (MALDI-TOF/TOF) to identify protein changes associated with the down-regulated expression of TIA proteins. We detected 30 differentially expressed proteins (DEPs), 24 of which were identified, and some of these DEPs were validated by western blotting. In silico analysis showed that DEPs were associated with metabolic processes, detoxification and proteostasis. We mapped the DEPs to the available biological pathways and networks, which included the metabolism of small molecules such as sugars, lipids, amino acids, and nucleotides. Our findings support previous studies and suggest that low expression of TIA proteins might act as a potential adaptive switch to link gene expression reprogramming to a proliferative phenotype mediated by a hormesis phenomenon.
- Subjects :
- Proteomics
0301 basic medicine
T-Lymphocytes
In silico
Biophysics
Biochemistry
Biological pathway
03 medical and health sciences
Hormesis
Gene expression
Animals
Humans
Electrophoresis, Gel, Two-Dimensional
Gene Regulatory Networks
Antigens
Molecular Biology
Chemistry
Gene Expression Profiling
Cell Biology
Cell biology
Blot
030104 developmental biology
Proteostasis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Proteome
Intracellular
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 503
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....0a631fc09c47dc949df65083ab2b6cfe