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High‐density adherent culture of CHO cells using rolled scaffold bioreactor

High‐density adherent culture of CHO cells using rolled scaffold bioreactor

Authors :
Ashkan YekrangSafakar
Ali Mehrnezhad
Tongyao Wu
Kidong Park
Source :
Biotechnology and Bioengineering. 119:1498-1508
Publication Year :
2022
Publisher :
Wiley, 2022.

Abstract

Rapid expansion of biopharmaceutical market calls for more efficient and reliable platforms to culture mammalian cells on a large scale. Stirred-tank bioreactors have been widely used for large-scale cell culture. However, it requires months of trials and errors to optimize culture conditions for each cell line. In this article, we extend our earlier studies on rolled scaffold (RS) bioreactors for high-density adherent cell culture and report two new implementations of RSs with greatly enhanced mass-manufacturability, termed as Mesh-RS and Fiber-RS. CHO-K1 cells were successfully expanded in Mesh-RS and Fiber-RS bioreactors with an average growth rate of 1.09 ± 0.04 1/day and 0.95 ± 0.07 1/day, which were higher than those reported in similar studies. Fiber-RS bioreactor exhibited a very high cell density of 72.8 × 10

Details

ISSN :
10970290 and 00063592
Volume :
119
Database :
OpenAIRE
Journal :
Biotechnology and Bioengineering
Accession number :
edsair.doi.dedup.....0a75f35113e696bb749a3d4c9a834d36
Full Text :
https://doi.org/10.1002/bit.28079