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Virus-triggered Ubiquitination of TRAF3/6 by cIAP1/2 Is Essential for Induction of Interferon-β (IFN-β) and Cellular Antiviral Response
- Source :
- Journal of Biological Chemistry. 285:9470-9476
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Viral infection causes activation of transcription factors NF-kappaB and IRF3, which collaborate to induce type I interferons (IFNs) and cellular antiviral response. Here we show that knockdown of the E3 ubiquitin ligases cIAP1 and cIAP2 markedly inhibited virus-triggered activation of IRF3 and NF-kappaB as well as IFN-beta induction. Knockdown of cIAP1 and cIAP2 also inhibited cytoplasmic dsRNA-triggered cellular antiviral response. Endogenous coimmunoprecipitation experiments indicated that viral infection caused recruitment of cIAP1 and cIAP2 to TRAF3, TRAF6, and VISA. Furthermore, we demonstrated that cIAP1- and cIAP2-mediated virus-triggered ubiquitination of TRAF3 and TRAF6. These findings suggest that virus-triggered ubiquitination of TRAF3 and TRAF6 by cIAP1 and cIAP2 is essential for type I IFN induction and cellular antiviral response.
- Subjects :
- TRAF3
Cytoplasm
Ubiquitin-Protein Ligases
viruses
Biology
Antiviral Agents
Biochemistry
Inhibitor of Apoptosis Proteins
Cytosol
Ubiquitin
Interferon
RNA interference
medicine
Humans
Molecular Biology
Transcription factor
RNA, Double-Stranded
TNF Receptor-Associated Factor 6
Gene knockdown
TNF Receptor-Associated Factor 3
NF-kappa B
Interferon-beta
Cell Biology
Molecular biology
Baculoviral IAP Repeat-Containing 3 Protein
Cell biology
biology.protein
RNA Interference
Signal transduction
IRF3
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 285
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....0aac7ba61bcb772777990c7bc857c58b