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Reconstructed β-Catenin/TCF4 Signaling in Yeast Applicable to Functional Evaluation of APC Mutations
- Source :
- The American Journal of Pathology. 163:2201-2209
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- In human genetics and molecular oncology, mutation research is necessary not only to identify mutations in nucleic acid sequences, but also to analyze the loss of function caused by mutant proteins. We reconstructed a protein-protein network system of human beta-catenin and TCF4, in Saccharomyces cerevisiae. beta-Catenin and TCF4 proteins form a complex and transactivate reporter genes. Co-expressed wild-type APC with beta-catenin and TCF4 inhibit the transcriptional activity of the beta-catenin/TCF4 complex in yeast, as well as in mammals. This unique method in which the beta-catenin/TCF4 signaling pathway is reconstructed in vivo may prove useful for the functional evaluation of APC mutants, including a type of APC truncated and missense mutants influenced to the ability of binding to beta-catenin.
- Subjects :
- Genes, APC
Transcription, Genetic
Tumor suppressor gene
Saccharomyces cerevisiae
Mutant
Mutation, Missense
Gene Expression
medicine.disease_cause
DNA-binding protein
Pathology and Forensic Medicine
medicine
Humans
Transcription factor
beta Catenin
Mutation
Reporter gene
biology
biology.organism_classification
Molecular biology
Cell biology
DNA-Binding Proteins
Cytoskeletal Proteins
Technical Advance
Catenin
Trans-Activators
Colorectal Neoplasms
TCF Transcription Factors
Transcription Factor 7-Like 2 Protein
Signal Transduction
Transcription Factors
Subjects
Details
- ISSN :
- 00029440
- Volume :
- 163
- Database :
- OpenAIRE
- Journal :
- The American Journal of Pathology
- Accession number :
- edsair.doi.dedup.....0ac4aef8df6f210fdf26d1aec8ce6aa5