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Solution NMR structure of CHU_1110 from Cytophaga hutchinsonii , an AHSA1 protein potentially involved in metal ion stress response
- Source :
- Proteins: Structure, Function, and Bioinformatics. 87:91-95
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- We report the solution nuclear magnetic resonance (NMR) structure of CHU_1110 from Cytophaga hutchinsonii. CHU_1110 contains three α-helices and one antiparallel β-sheet, forming a large cavity in the center of the protein, which are consistent with the structural characteristics of AHSA1 protein family. This protein shows high structural similarities to the prokaryotic proteins RHE_CH02687 from Rhizobium etli and YndB from Bacillus subtilis, which can bind with flavinoids. Unlike these two homologs, CHU_1110 shows no obvious interaction with flavonoids in NMR titration experiments. In addition, no direct interaction has been observed between CHU_1110 and ATP, although many homologous sequences of CHU_1110 have been annotated as ATPase. Combining the analysis of structural similarity of CHU_1110 and genomic context of its encoding gene, we speculate that CHU_1110 may be involved in the stress response of bacteria to heavy metal ions, even though its specific biological functions that need to be further investigated.
- Subjects :
- Models, Molecular
Protein family
Protein Conformation
Stereochemistry
Structural similarity
ATPase
Bacillus subtilis
Cytophaga
Antiparallel (biochemistry)
Biochemistry
03 medical and health sciences
Bacterial Proteins
Stress, Physiological
Structural Biology
Rhizobium etli
Humans
Nuclear Magnetic Resonance, Biomolecular
Molecular Biology
Gene
030304 developmental biology
0303 health sciences
biology
Chemistry
030302 biochemistry & molecular biology
biology.organism_classification
Metals
biology.protein
Bacteria
Molecular Chaperones
Subjects
Details
- ISSN :
- 08873585
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Proteins: Structure, Function, and Bioinformatics
- Accession number :
- edsair.doi.dedup.....0ac786124fa9023786011893717cb5e4