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Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-gamma production by up-regulating T-bet expression in patients with asthma
- Source :
- Chinese journal of integrative medicine. 12(4)
- Publication Year :
- 2007
-
Abstract
- To explore the effect of Astragalus membranaceus (AM) on T-helper cell type 1 (Thl) specific transcription factor T-box expressed in T cells (T-bet) expression and Thl/Th2 equilibrium.The levels of T-bet mRNA in peripheral blood mononuclear cells (PBMCs) from 15 patients with asthma and 15 healthy subjects were determined by reverse transcription-polymerase chain reaction (RT-PCR). PBMCs in asthma patients were incubated with AM and then the concentration of interferon gamma (IFN-gamma) and interleukin-4 (IL-4) in the supernate before and after AM intervention were determined by ELISA. The numbers of CD4 + CCR3 + and CD4 + CCR5 + cells were counted by flow cytometry.The expression of T-bet mRNA and the level of IFN-gamma were lower, but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 microg/ml) intervention, the former two parameters raised and showed a positive correlation between them, while the level of IL-4 was decreased. The mean percentage of CD4 + CCR3 + cells in asthma patients was significantly higher but that of CD4 + CCR5 + cells was lower when compared with those in healthy subjects respectively. After AM intervention, the abnormal change in the two indexes was improved to certain extent, showing a reversing status of Th2 polarization.AM could increase the expression of T-bet mRNA and Thl cytokines such as IFN-Y, and might reverse the Th2 predominant status in asthma patients.
- Subjects :
- Adult
Male
Cell type
Receptors, CCR5
Receptors, CCR3
CCR3
Interferon-gamma
Downregulation and upregulation
medicine
Humans
Pharmacology (medical)
RNA, Messenger
Receptor
Transcription factor
biology
Cell Polarity
General Medicine
T helper cell
Astragalus propinquus
Middle Aged
Th1 Cells
biology.organism_classification
Molecular biology
Asthma
Up-Regulation
Astragalus
medicine.anatomical_structure
Cross-Sectional Studies
Complementary and alternative medicine
Immunology
Female
Receptors, Chemokine
Interleukin-4
T-Box Domain Proteins
Phytotherapy
Subjects
Details
- ISSN :
- 16720415
- Volume :
- 12
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Chinese journal of integrative medicine
- Accession number :
- edsair.doi.dedup.....0ae669d147e07f810d62e26c3bec0d1d